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Plant and Cell Physiology Advance Access originally published online on November 23, 2007
Plant and Cell Physiology 2008 49(1):126-132; doi:10.1093/pcp/pcm164
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

Analysis of GDP-D-Mannose Pyrophosphorylase Gene Promoter from Acerola (Malpighia glabra) and Increase in Ascorbate Content of Transgenic Tobacco Expressing the Acerola Gene

Adebanjo A. Badejo1, Nobukazu Tanaka2 and Muneharu Esaka1,*

1Graduate School of Biosphere Sciences, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-8528 Japan
2Center for Gene Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-8527 Japan

*Corresponding author: E-mail, mesaka{at}hiroshima-u.ac.jp; Fax, +81-82-424-7916.


  Abstract

GDP-D-mannose pyrophosphorylase (GMP) is an important enzyme in the Smirnoff–Wheeler's pathway for the biosynthesis of ascorbic acid (AsA) in plants. We have reported recently that the expression of the acerola (Malpighia glabra) GMP gene, designated MgGMP, correlates with the AsA content of the plant. The acerola plant has very high levels of AsA relative to better studied model plants such as Arabidopsis. Here we found that the GMP mRNA levels in acerola are higher than those from Arabidopsis and tomato. Also, the transient expression of the uidA reporter gene in the protoplasts of Nicotiana tabacum cultures showed the MgGMP gene promoter to have higher activity than the cauliflower mosaic virus 35S and Arabidopsis GMP promoters. The AsA content of transgenic tobacco plants expressing the MgGMP gene including its promoter was about 2-fold higher than that of the wild type.

Keywords: Acerola (Malpighia glabra) - Ascorbic acid - GDP-D-mannose pyrophosphorylase - Promoter analysis - Protoplast - Transgenic tobacco

Abbreviations: AsA, ascorbic acid; CaMV, cauliflower mosaic virus; GMP, GDP-D-mannose pyrophosphorylase; GUS (uidA), β-glucuronidase; MES, 2-morpholinoethanesulfonic acid monohydrate; MgGMP, Malpighia glabra GDP-D-mannose pyrophosphorylase; npt II, neomycin phosphotransferase gene; PEG, polyethylene glycol; RACE, rapid amplification of cDNA ends; TSS, transcription start site

(Received October 8, 2007; Accepted November 21, 2007)
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