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Plant and Cell Physiology Advance Access originally published online on August 22, 2007
Plant and Cell Physiology 2007 48(9):1385-1390; doi:10.1093/pcp/pcm106
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

Cyanobacteriochrome TePixJ of Thermosynechococcus elongatus Harbors Phycoviolobilin as a Chromophore

Takami Ishizuka1, Rei Narikawa1, Takayuki Kohchi2, Mitsunori Katayama1 and Masahiko Ikeuchi1,*

1Department of Life Sciences (Biology), The University of Tokyo, Komaba 3-8-1, Meguro, Tokyo, 153-8902 Japan
2Graduate School of Biostudies, Kyoto University, Kitashirakawa-oiwake, Sakyo, Kyoto, 606-8502 Japan

*Corresponding author: E-mail, mikeuchi{at}bio.c.u-tokyo.ac.jp; Fax, +81-3-5454-4337.


  Abstract

Cyanobacteria have several putative photoreceptors (designated cyanobacteriochromes) that are related to but distinct from the established phytochromes. The GAF domain of the phototaxis regulator, PixJ, from a thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 (TePixJ_GAF) is a cyanobacteriochrome which exhibits reversible photoconversion between a blue light-absorbing form (max = 433 nm) and a green light-absorbing form (max = 531 nm). To study the chromophore, we prepared TePixJ_GAF chromoprotein from heterologously expressed Synechocystis and performed spectral analysis after denaturation by comparing it with the cyanobacterial phytochrome Cph1 which harbors phycocyanobilin (PCB) as a chromophore. The results indicated that the chromophore of TePixJ is not PCB, but its isomer, phycoviolobilin (PVB). It is suggested that the GAF domain of TePixJ has auto-lyase and auto-isomerase activities.

Keywords: Auto-isomerase — Cyanobacteria — Cyanobacteriochrome — Phycocyanobilin — Phycoviolobilin — Phytochrome

Abbreviations: LED, light-emitting diode; PCB, phycocyanobilin; PEC, phycoerythrocyanin; PVB, phycoviolobilin; Pb, blue light-absorbing form; Pg, green light-absorbing form.

(Received May 12, 2007; Accepted August 8, 2007)
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