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Plant and Cell Physiology Advance Access originally published online on July 24, 2007
Plant and Cell Physiology 2007 48(9):1275-1290; doi:10.1093/pcp/pcm094
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

CRM1/BIG-Mediated Auxin Action Regulates Arabidopsis Inflorescence Development

Nobutoshi Yamaguchi1, Mitsuhiro Suzuki1, Hidehiro Fukaki2,3, Miyo Morita-Terao2, Masao Tasaka2 and Yoshibumi Komeda1,*

1Laboratory of Plant Science, Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033 Japan
2Graduate School of Biological Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0101 Japan

*Corresponding author: E-mail, komeda-y{at}biol.s.u-tokyo.ac.jp; Fax, +81-3-5841-4454.


  Abstract

The shape of the inflorescence in Arabidopsis thaliana ecotype Columbia is a raceme with individual flowers developing acropetally. The ecotype Landsberg harboring the erecta (er) mutation shows a corymb-like inflorescence, namely a compact inflorescence with a flattened arrangement of flower buds at the tip. To gain insight into inflorescence development, we previously isolated corymb-like inflorescence mutants, named corymbosa1 (crm1), and found that the corymb-like inflorescence in crm1-1 was due to reduced cell elongation of pedicels and stem internodes. Double mutants of crm1 with er and crm2, and crm1-1 crm2-1 er-105 triple mutants show an additive phenotype. crm1-1 is caused by a mutation in BIG, which is required for polar auxin transport. CRM1/BIG is expressed in inflorescence meristems, floral meristems and vascular tissues. We analyzed a collection of 12 reduced lateral root formation (rlr) mutants, which are allelic to crm1-1, and categorized the mutants into three classes, depending on the plant developmental defects. Although all 12 alleles had new stop codons, the phenotype of heterozygous crm1-1/doc1-1 and Northern blotting suggest that new crm1/big mutant alleles are hypomorphic. Auxin-responsive DR5rev::GFP expression was decreased in crm1-1 vasculature of pedicels and stem internodes. PINFORMED1 (PIN1) and CRM1/BIG are expressed in vasculature of pedicels and stem internodes. The severity of corymb-like inflorescence in crm1/big mutants correlated with increased levels of PIN1. Our results suggest that CRM1/BIG controls the elongation of the pedicels and stem internodes through auxin action.

Keywords: Arabidopsis thaliana - BIG gene - Corymb-like inflorescence - corymbosa1 - Inflorescence development - Polar auxin transport

Abbreviations: CAPS, cleaved amplified polymorphic sequence; CRD, cysteine-rich putative zinc-finger domain; EMS, ethylmethane sulfonate; DR5, direct repeat 5; GFP, green fluorescent protein; GUS, ß-glucuronidase; NAA, 1-naphthaleneacetic acid; SEM, reverse transcription–PCR; GUS, scanning electron microscopy

3Present address: Graduate School of Science, Kobe University, 1-1 Rokkodai, Kobe, 657-8501, Japan.

(Received May 18, 2007; Accepted July 14, 2007)
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