Skip Navigation


Plant and Cell Physiology Advance Access originally published online on July 18, 2007
Plant and Cell Physiology 2007 48(8):1081-1091; doi:10.1093/pcp/pcm091
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary data
Right arrow All Versions of this Article:
48/8/1081    most recent
pcm091v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (23)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Furukawa, J.
Right arrow Articles by Ma, J. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Furukawa, J.
Right arrow Articles by Ma, J. F.
Agricola
Right arrow Articles by Furukawa, J.
Right arrow Articles by Ma, J. F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Rapid Paper

An Aluminum-Activated Citrate Transporter in Barley

Jun Furukawa1, Naoki Yamaji1, Hua Wang1, Namiki Mitani1, Yoshiko Murata2, Kazuhiro Sato1, Maki Katsuhara1, Kazuyoshi Takeda1 and Jian Feng Ma1,*

1Research Institute for Bioresources, Okayama University, Chuo, Kurashiki, Okayama, 710-0046 Japan
2Suntory Institute for Bioorganic Research, Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka, 618-8503 Japan

*Corresponding author: E-mail, maj{at}rib.okayama-u.ac.jp; Fax, +81-86-434-1209.


  Abstract

Soluble ionic aluminum (Al) inhibits root growth and reduces crop production on acid soils. Al-resistant cultivars of barley (Hordeum vulgare L.) detoxify Al by secreting citrate from the roots, but the responsible gene has not been identified yet. Here, we identified a gene (HvAACT1) responsible for the Al-activated citrate secretion by fine mapping combined with microarray analysis, using an Al-resistant cultivar, Murasakimochi, and an Al-sensitive cultivar, Morex. This gene belongs to the multidrug and toxic compound extrusion (MATE) family and was constitutively expressed mainly in the roots of the Al-resistant barley cultivar. Heterologous expression of HvAACT1 in Xenopus oocytes showed efflux activity for 14C-labeled citrate, but not for malate. Two-electrode voltage clamp analysis also showed transport activity of citrate in the HvAACT1-expressing oocytes in the presence of Al. Overexpression of this gene in tobacco enhanced citrate secretion and Al resistance compared with the wild-type plants. Transiently expressed green fluorescent protein-tagged HvAACT1 was localized at the plasma membrane of the onion epidermal cells, and immunostaining showed that HvAACT1 was localized in the epidermal cells of the barley root tips. A good correlation was found between the expression of HvAACT1 and citrate secretion in 10 barley cultivars differing in Al resistance. Taken together, our results demonstrate that HvAACT1 is an Al-activated citrate transporter responsible for Al resistance in barley.

Keywords: Aluminum - Barley - Citrate transporter - MATE - Resistance - Root

Abbreviations: BAC, bacterial artificial chromosome; CaMV, cauliflower mosaic virus; EST, expressed sequence tag; GFP, green fluorescent protein; MATE, multidrug and toxic compound extrusion; ORF, open reading frame; QTL, quantitative trait locus; SNP, single nucleotide polymorphism

(Received June 26, 2007; Accepted July 9, 2007)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
C. Gomez, N. Terrier, L. Torregrosa, S. Vialet, A. Fournier-Level, C. Verries, J.-M. Souquet, J.-P. Mazauric, M. Klein, V. Cheynier, et al.
Grapevine MATE-Type Proteins Act as Vacuolar H+-Dependent Acylated Anthocyanin Transporters
Plant Physiology, May 1, 2009; 150(1): 402 - 415.
[Abstract] [Full Text] [PDF]

Home page
 DNA ResHome page
K. Sato, T. Shin-I, M. Seki, K. Shinozaki, H. Yoshida, K. Takeda, Y. Yamazaki, M. Conte, and Y. Kohara
Development of 5006 Full-Length CDNAs in Barley: A Tool for Accessing Cereal Genomics Resources
DNA Res, April 1, 2009; 16(2): 81 - 89.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Morita, N. Shitan, K. Sawada, M. C. E. Van Montagu, D. Inze, H. Rischer, A. Goossens, K.-M. Oksman-Caldentey, Y. Moriyama, and K. Yazaki
Vacuolar transport of nicotine is mediated by a multidrug and toxic compound extrusion (MATE) transporter in Nicotiana tabacum
PNAS, February 17, 2009; 106(7): 2447 - 2452.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C. F. Huang, N. Yamaji, N. Mitani, M. Yano, Y. Nagamura, and J. F. Ma
A Bacterial-Type ABC Transporter Is Involved in Aluminum Tolerance in Rice
PLANT CELL, February 1, 2009; 21(2): 655 - 667.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. R. Ryan, H. Raman, S. Gupta, W. J. Horst, and E. Delhaize
A Second Mechanism for Aluminum Resistance in Wheat Relies on the Constitutive Efflux of Citrate from Roots
Plant Physiology, January 1, 2009; 149(1): 340 - 351.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Yokosho, N. Yamaji, D. Ueno, N. Mitani, and J. F. Ma
OsFRDL1 Is a Citrate Transporter Required for Efficient Translocation of Iron in Rice
Plant Physiology, January 1, 2009; 149(1): 297 - 305.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
W.-H. Zhang, P. R. Ryan, T. Sasaki, Y. Yamamoto, W. Sullivan, and S. D. Tyerman
Characterization of the TaALMT1 Protein as an Al3+-Activated Anion Channel in Transformed Tobacco (Nicotiana tabacum L.) Cells
Plant Cell Physiol., September 1, 2008; 49(9): 1316 - 1330.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. C. Collins, F. Tardieu, and R. Tuberosa
Quantitative Trait Loci and Crop Performance under Abiotic Stress: Where Do We Stand?
Plant Physiology, June 1, 2008; 147(2): 469 - 486.
[Full Text] [PDF]

Home page
 GeneticsHome page
N. C. Collins, N. J. Shirley, M. Saeed, M. Pallotta, and J. P. Gustafson
An ALMT1 Gene Cluster Controlling Aluminum Tolerance at the Alt4 Locus of Rye (Secale cereale L.)
Genetics, May 1, 2008; 179(1): 669 - 682.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.