Skip Navigation


Plant and Cell Physiology Advance Access originally published online on June 6, 2007
Plant and Cell Physiology 2007 48(7):1036-1049; doi:10.1093/pcp/pcm069
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary data
Right arrow An erratum has been published
Right arrow All Versions of this Article:
48/7/1036    most recent
pcm069v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (3)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Tanabe, N.
Right arrow Articles by Shigeoka, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tanabe, N.
Right arrow Articles by Shigeoka, S.
Agricola
Right arrow Articles by Tanabe, N.
Right arrow Articles by Shigeoka, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Differential Expression of Alternatively Spliced mRNAs of Arabidopsis SR Protein Homologs, atSR30 and atSR45a, in Response to Environmental Stress

Noriaki Tanabe1, Kazuya Yoshimura2, Ayako Kimura1, Yukinori Yabuta1 and Shigeru Shigeoka1,*

1Advanced Bioscience, Graduate School, Kinki University, 3327-204 Nakamachi, Nara, 631-8505 Japan
2Department of Food and Nutritional Science, College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi, 487-8501 Japan

*Corresponding author: E-mail, shigeoka{at}nara.kindai.ac.jp; Fax, +81-742-43-8976.


  Abstract

Serine/arginine-rich (SR) proteins are associated with either the regulation or the execution of both constitutive splicing and the selection of alternative splice sites in animals and plants. We demonstrated the molecular characterization of a homolog of SR protein, atSR45a, in Arabidopsis plants. Six types of mRNA variants (atSR45a-1a–e and atSR45a-2) were generated by the alternative selection of transcriptional initiation sites and the alternative splicing of introns in atSR45a pre-mRNA. The atSR45a-1a and -2 proteins, presumed mature forms, were located in the nucleus and interacted with U1-70K, suggesting that these proteins function as a splicing factor in Arabidopsis. The levels of the transcripts atSR45a and atSR30, SF2/ASF-like SR proteins, were increased by various types of stress, such as high-light irradiation and salinity. Furthermore, the splicing patterns of atSR45a and atSR30 pre-mRNA themselves were altered under these stressful conditions. In particular, the expression of atSR45a-1a, atSR45a-2, atSR30 mRNA1 and atSR30 mRNA3 was greatly increased by high-light irradiation. These results indicate that the regulation of transcription and alternative splicing of atSR45a and atSR30 is responsive to various stressful conditions.

Keywords: Alternative splicing - Arabidopsis - High-light irradiation - SR protein

Abbreviations: CaMV 35S, cauliflower mosaic virus 35S promoter; DAPI, 4',6-diamidino-2-phenylindole dihydrochloride; GFP, green fluorescent protein; NMD, nonsense-mediated mRNA decay; ORF, open reading frame; PQ, paraquat; PTC, premature termination codon; RT–PCR, reverse transcriptase–PCR; RRM, RNA-recognition motif; snRNP, small nuclear ribonucleoprotein particle; SR, serine/arginine-rich; UTR, untranslated region.

(Received April 20, 2007; Accepted June 5, 2007)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 RNAHome page
Q. M. Mitrovich and C. Guthrie
Evolution of small nuclear RNAs in S. cerevisiae, C. albicans, and other hemiascomycetous yeasts
RNA, December 1, 2007; 13(12): 2066 - 2080.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.