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Plant and Cell Physiology Advance Access originally published online on April 27, 2007
Plant and Cell Physiology 2007 48(6):812-821; doi:10.1093/pcp/pcm051
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Stable Oil Bodies Sheltered by a Unique Oleosin in Lily Pollen

Pei-Luen Jiang1, Co-Shing Wang1, Chia-Mei Hsu2, Guang-Yuh Jauh2,* and Jason T. C. Tzen1,3,*

1Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan
2Institute of Plant and Microbial Biology, Academia Sinica, Taipei 115, Taiwan
3Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan

*Corresponding authors: Guang-Yuh Jauh, E-mail, jauh{at}gate.sinica.edu.tw; Fax, +886-4-22853527; Jason T. C. Tzen, E-mail, TCTZEN{at}dragon.nchu.edu.tw; Fax, +886-4-22853527.


  Abstract

Stable oil bodies were purified from mature lily (Lilium longiflorum Thunb.) pollen. The integrity of pollen oil bodies was maintained via electronegative repulsion and steric hindrance possibly provided by their surface proteins. Immunodetection revealed that a major protein of 18 kDa was exclusively present in pollen oil bodies and massively accumulated in late stages of pollen maturation. According to mass spectrometric analyses, this oil body protein possessed a tryptic fragment of 13 residues matching that of a theoretical rice oleosin. A complete cDNA fragment encoding this putative oleosin was obtained by PCR cloning with primers derived from its known 13-residue sequence. Sequence analysis as well as immunological non-cross-reactivity suggests that this pollen oleosin represents a distinct class in comparison with oleosins found in seed oil bodies and tapetum. In pollen cells observed by electron microscopy, oil bodies were presumably surrounded by tubular membrane structures, and encapsulated in the vacuoles after germination. It seems that pollen oil bodies are mobilized via a different route from that of glyoxysomal mobilization of seed oil bodies after germination.

Keywords: Germination - Lily (Lilium longiflorum Thunb.) - Oil bodies - Oleosin - Pollen

Abbreviations: MALDI-MS, matrix-assisted laser desorption/ionization-mass spectrometry; PL, phospholipid; TAG, triacylglycerol

(Received March 17, 2007; Accepted April 20, 2007)
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