Skip Navigation


Plant and Cell Physiology Advance Access originally published online on April 26, 2007
Plant and Cell Physiology 2007 48(6):804-811; doi:10.1093/pcp/pcm048
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
48/6/804    most recent
pcm048v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (3)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Hanana, M.
Right arrow Articles by Blumwald, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hanana, M.
Right arrow Articles by Blumwald, E.
Agricola
Right arrow Articles by Hanana, M.
Right arrow Articles by Blumwald, E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

A Grape Berry (Vitis vinifera L.) Cation/Proton Antiporter is Associated with Berry Ripening

Mohsen Hanana1,2, Olivier Cagnac2, Toshio Yamaguchi2, Saïd Hamdi3, Abdelwahed Ghorbel1 and Eduardo Blumwald2,*

1Laboratoire de Physiologie Moléculaire de la Vigne, INRST, BP 901, Hammam-lif 2050, Tunisia
2Department of Plant Sciences, University of California, One Shields Ave, Davis, CA 95616, USA
3Laboratoire de Génomique Fonctionnelle et de la Qualité de la Baie de Raisin, Université de Bordeaux 1, 71 Av. Edouard Bourleaux, BP 81, 33883Bordeaux, France

*Corresponding author: E-mail, eblumwald{at}ucdavis.edu; Fax, +1-530-752-2278.


  Abstract

We have cloned and characterized VvNHX1, a gene encoding a vacuolar cation/H+ antiporter from Vitis vinifera cv. Cabernet Sauvignon. VvNHX1 belongs to the vacuolar NHX protein family and showed high similarity to other known vacuolar antiporters. The expression of VvNHX1 partially complements the salt- and hygromycin-sensitive phenotypes of an ena1-4 nhx1 yeast strain. Immunoblots of vacuoles of yeast expressing a VvNHX1, together with the expression of a VvNHX1–GFP (green fluorescent protein) chimera demonstrated that VvNHX1 localized to the vacuoles. VvNHX1 displayed low affinity K+/H+ and Na+/H+ exchange activities (12.8 and 40.2 mM, respectively). The high levels of expression of VvNHX1 during the véraison and post-véraison stages would indicate that the increase in vacuolar K+ accumulation, mediated by VvNHX1, is needed for vacuolar expansion. This process, together with the rapid accumulation of reducing sugars, would drive water uptake to the berry and the concomitant berry size increase, typical of the post-véraison stage of growth.

Keywords: Cation/H+ antiporter - Grape berry — NHX - Vacuole - Véraison - Vitis vinifera

Abbreviations: EST, expressed sequence tag; GFP, green fluorescent protein; ORF, open reading frame; RT–PCR, reverse transcription–PCR

(Received March 13, 2007; Accepted April 19, 2007)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
F. Queiros, N. Fontes, P. Silva, D. Almeida, M. Maeshima, H. Geros, and F. Fidalgo
Activity of tonoplast proton pumps and Na+/H+ exchange in potato cell cultures is modulated by salt
J. Exp. Bot., March 1, 2009; 60(4): 1363 - 1374.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.