Plant and Cell Physiology Advance Access originally published online on March 26, 2007
Plant and Cell Physiology 2007 48(5):715-723; doi:10.1093/pcp/pcm039
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Nitric Oxide Inhibits Blue Light-Specific Stomatal Opening Via Abscisic Acid Signaling Pathways in Vicia Guard Cells
1Department of Biology, Faculty of Science, Kyushu University, Ropponmatsu, Fukuoka, 810-8560 Japan
2Henan Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Kaifeng 475001, PR China
*Corresponding author: E-mail, kenrcb{at}mbox.nc.kyushu-u.ac.jp; Fax, +81-92-726-4758.
 |
Abstract |
|---|
Recent evidence suggests that nitric oxide (NO) acts as an intermediate of ABA signal transduction for stomatal closure. However, NO's effect on stomatal opening is poorly understood even though both opening and closing activities determine stomatal aperture. Here we show that NO inhibits stomatal opening specific to blue light, thereby stimulating stomatal closure. NO inhibited blue light-specific stomatal opening but not red light-induced opening. NO inhibited both blue light-induced H+ pumping and H+-ATPase phosphorylation. The NO scavenger 2-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO) restored all these inhibitory effects. ABA and hydrogen peroxide (H2O2) inhibited all of these blue light-specific responses in a manner similar to NO. c-PTIO partially restored the ABA-induced inhibition of all of these opening responses but did not restore inhibition of the responses by H2O2. ABA, H2O2 and NO had slight inhibitory effects on the phosphorylation of phototropins, which are blue light receptors in guard cells. NO inhibited neither fusicoccin-induced H+ pumping in guard cells nor H+ transport by H+-ATPase in the isolated membranes. From these results, we conclude that both NO and H2O2 inhibit blue light-induced activation of H+-ATPase by inhibiting the component(s) between phototropins and H+-ATPase in guard cells and stimulate stomatal closure by ABA.
Keywords: Abscisic acid - Blue light - H+ pumping - Nitric oxide - Stomatal opening
Abbreviations: c-PTIO, 2-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide; NO, nitric oxide; SNP, sodium nitroprusside; Vfphot, Vicia faba phototropin.
(Received February 5, 2007; Accepted March 20, 2007)
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Sirichandra, A. Wasilewska, F. Vlad, C. Valon, and J. Leung The guard cell as a single-cell model towards understanding drought tolerance and abscisic acid action J. Exp. Bot., April 1, 2009; 60(5): 1439 - 1463. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Asai, K. Ohta, and H. Yoshioka MAPK Signaling Regulates Nitric Oxide and NADPH Oxidase-Dependent Oxidative Bursts in Nicotiana benthamiana PLANT CELL, May 1, 2008; 20(5): 1390 - 1406. [Abstract] [Full Text] [PDF]
|
|