Plant and Cell Physiology Advance Access originally published online on February 28, 2007
Plant and Cell Physiology 2007 48(4):598-605; doi:10.1093/pcp/pcm032
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Spectral and Dose Dependence of Light-Induced Ion Flux Responses from Maize Leaves and their Involvement in Leaf Expansion Growth
an 
ivanovi
1,2
1School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart, Tasmania 7001, Australia
2Center for Multidisciplinary Studies, University of Belgrade, Bulevar despota Stefana 142, 10060 Belgrade, Serbia
*Corresponding author: E-mail, Sergey.Shabala{at}utas.edu.au; Fax, +61-3-6226-2642.
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Abstract |
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Two types of segments (intact leaf tissue and isolated mesophyll tissue respectively) were isolated from basal (still growing) and tip (non-growing) maize leaf regions. The leaf segments were exposed to different light qualities (blue or red light) and quantities, and net fluxes of K+, Ca2+ and H+ were measured non-invasively using ion-selective vibrating microelectrodes (the MIFE technique). A clear dose dependency of all ion flux responses on both red (RL) and blue (BL) light fluence rate was found. We provide evidence that light-induced K+ flux kinetics are different between growing and non-growing tissues and attribute this difference to the direct involvement of RL-induced K+ flux in turgor-driven leaf expansion growth controlled by the epidermis, as well as to the charge-balancing role of K+ in the leaf mesophyll. Generally, BL was much more efficient in stimulating K+ uptake in the growing basal region compared with RL. We also show a much stronger influence of RL on Ca2+ fluxes in the basal region compared with BL, which argues in favor of the importance of RL in Ca2+ signaling during leaf growth.
Keywords: Expansion growth - Ion fluxes - Ion-selective microelectrodes - Light - Photosynthesis - Zea mays L
Abbreviations: BL, blue light; MIFE, microelectrode ion flux estimation; RL, red light; WL, white light
(Received January 17, 2007; Accepted February 21, 2007)
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