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Plant and Cell Physiology Advance Access originally published online on January 3, 2007
Plant and Cell Physiology 2007 48(2):369-374; doi:10.1093/pcp/pcl064
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

Characterization of a Splicing Variant of Plant Aurora Kinase

Daisuke Kurihara1,2, Akira Kawabe1,2,3, Sachihiro Matsunaga1, Katsuyuki Nakagawa1, Satoru Fujimoto1,4, Susumu Uchiyama1 and Kiichi Fukui1,*

1Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871 Japan

*Corresponding author: E-mail, kfukui{at}bio.eng.osaka-u.ac.jp; Fax, +81-6-6879-7441.


  Abstract

Aurora kinases play a key role in chromosome segregation and cytokinesis. In plants, three Aurora kinases (AtAUR1–AtAUR3) have been identified in Arabidopsis thaliana. Here, we report an AtAUR2 splicing variant (AtAUR2S), which lacks the fourth exon encoding a part of the kinase domain of AtAUR2. AtAUR2S was shown to have lost its kinase activity to phosphorylate histone H3 at Ser10; however, it maintained its ability to bind to histone H3. The localization pattern of AtAUR2S was the same as that of AtAUR2. The findings suggest that AtAUR2S affects cell division by competing with AtAUR2.

Keywords: Alternative splicing - Arabidopsis thaliana - Aurora kinase - Histone H3

Abbreviations: BSA, bovine serum albumin; DAPI, 4',6-diamidino-2-phenylindole; GFP, green fluorescent protein; GST, glutathione-S-transferase; PBS, phosphate-buffered saline; RT–PCR, reverse transcription–PCR

2 These authors contributed equally to this work.

3 Present address: Institute of Evolutionary Biology, University of Edinburgh, Ashworth Laboratory, King's Buildings, West Mains Road, Edinburgh EH9 3JT, UK.

4 Present address: Department of Molecular Biology 3944, College of Agriculture, University of Wyoming, 1000 East University Avenue, Laramie, WY 82071, USA.

(Received November 18, 2006; Accepted December 24, 2006)
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D. Kurihara, S. Matsunaga, S. Uchiyama, and K. Fukui
Live Cell Imaging Reveals Plant Aurora Kinase Has Dual Roles During Mitosis
Plant Cell Physiol., August 1, 2008; 49(8): 1256 - 1261.
[Abstract] [Full Text] [PDF]


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