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Plant and Cell Physiology Advance Access originally published online on November 7, 2007
Plant and Cell Physiology 2007 48(12):1803-1808; doi:10.1093/pcp/pcm153
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

The Major Route for Chlorophyll Synthesis Includes [3,8-divinyl]-chlorophyllide a Reduction in Arabidopsis thaliana

Nozomi Nagata1,2,4,*, Ryouichi Tanaka1,3 and Ayumi Tanaka1,3

1 Institute of Low Temperature Science, Hokkaido University, Sapporo, 060-0819 Japan
2 Japan Society for the Promotion of Science (JSPS), Tokyo, 102-8472 Japan
3 CREST, Japan Science and Technology Corporation, Japan

*Corresponding author: E-mail, nagata{at}lowtem.hokudai.ac.jp; Fax, +81-11-706-5493.


  Abstract

In most reviews on Chl biosynthesis, Chl is described as being synthesized via the route involving the reduction of [3,8-divinyl]-protochlorophyllide a. However, the possibility remains that the conversion of the divinyl form of the Chl intermediate to its monovinyl form takes place at other enzymatic steps. To determine the actual route of Chl biosynthesis, we examined the substrate specificity of the formerly named [3,8-divinyl]-protochlorophyllide a 8-vinyl reductase (DVR) in vitro. In addition, we investigated the accumulation of various Chl intermediates in etiolated seedlings in vivo. Collectively, these studies indicate that [3,8-divinyl]-chlorophyllide a is the major substrate of DVR.

Keywords: Arabidopsis thaliana - Chlorophyll biosynthetic pathway - [3,8-Divinyl]-protochlorophyllide a - [3,8-Divinyl]-chlorophyllide a 8-vinyl reductase

Abbreviations: Chlide, chlorophyllide; DV, divinyl; DVR, [3,8-divinyl]-chlorophyllide a 8-vinyl reductase; MV, monovinyl; POR, Pchlide, protochlorophyllide.

4Present address: Laboratory of Molecular and Celluler Biology of Totipotency, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, 606-8502 Japan.

(Received October 3, 2007; Accepted November 5, 2007)
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