Plant and Cell Physiology Advance Access originally published online on November 7, 2007
Plant and Cell Physiology 2007 48(12):1803-1808; doi:10.1093/pcp/pcm153
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The Major Route for Chlorophyll Synthesis Includes [3,8-divinyl]-chlorophyllide a Reduction in Arabidopsis thaliana
1 Institute of Low Temperature Science, Hokkaido University, Sapporo, 060-0819 Japan
2 Japan Society for the Promotion of Science (JSPS), Tokyo, 102-8472 Japan
3 CREST, Japan Science and Technology Corporation, Japan
*Corresponding author: E-mail, nagata{at}lowtem.hokudai.ac.jp; Fax, +81-11-706-5493.
| Abstract |
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In most reviews on Chl biosynthesis, Chl is described as being synthesized via the route involving the reduction of [3,8-divinyl]-protochlorophyllide a. However, the possibility remains that the conversion of the divinyl form of the Chl intermediate to its monovinyl form takes place at other enzymatic steps. To determine the actual route of Chl biosynthesis, we examined the substrate specificity of the formerly named [3,8-divinyl]-protochlorophyllide a 8-vinyl reductase (DVR) in vitro. In addition, we investigated the accumulation of various Chl intermediates in etiolated seedlings in vivo. Collectively, these studies indicate that [3,8-divinyl]-chlorophyllide a is the major substrate of DVR.
Keywords: Arabidopsis thaliana - Chlorophyll biosynthetic pathway - [3,8-Divinyl]-protochlorophyllide a - [3,8-Divinyl]-chlorophyllide a 8-vinyl reductase
Abbreviations: Chlide, chlorophyllide; DV, divinyl; DVR, [3,8-divinyl]-chlorophyllide a 8-vinyl reductase; MV, monovinyl; POR, Pchlide, protochlorophyllide.
4Present address: Laboratory of Molecular and Celluler Biology of Totipotency, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, 606-8502 Japan.
(Received October 3, 2007; Accepted November 5, 2007)
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