Skip Navigation


Plant and Cell Physiology Advance Access originally published online on October 4, 2007
Plant and Cell Physiology 2007 48(11):1652-1657; doi:10.1093/pcp/pcm125
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
48/11/1652    most recent
pcm125v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Shimamura, M.
Right arrow Articles by Aoki, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shimamura, M.
Right arrow Articles by Aoki, T.
Agricola
Right arrow Articles by Shimamura, M.
Right arrow Articles by Aoki, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

2-Hydroxyisoflavanone Dehydratase is a Critical Determinant of Isoflavone Productivity in Hairy Root Cultures of Lotus japonicus

Masayuki Shimamura1, Tomoyoshi Akashi1, Nozomu Sakurai2, Hideyuki Suzuki2, Kazuki Saito3, Daisuke Shibata2, Shin-ichi Ayabe1 and Toshio Aoki1,*

1Department of Applied Biological Sciences, Nihon University, Fujisawa, Kanagawa, 252-8510 Japan
2Kazusa DNA Research Institute, Kisarazu, Chiba, 292-0818 Japan and
3Graduate School of Pharmaceutical Sciences, Chiba University, Chiba, 263-8522 Japan

*Corresponding author: E-mail, taoki{at}brs.nihon-u.ac.jp; Fax, +81-466-84-3353.


  Abstract

Hairy root cultures of a model legume, Lotus japonicus, were established to characterize two heterologous cDNAs encoding enzymes involved in isoflavone biosynthesis, i.e. licorice 2-hydroxyisoflavanone synthase (IFS) and soybean 2-hydroxyisoflavanone dehydratase (HID) catalyzing sequential reactions to yield isoflavones. While the control and the IFS overexpressor did not accumulate detectable isoflavones, the HID overexpressors did accumulate daidzein and genistein, showing that HID is a critical determinant of isoflavone productivity. Production of coumestrol in all the genotypes and isoliquiritigenin/liquiritigenin in IFS + HID-overexpressing lines was also noted. These results provide insight into the regulatory mechanism that controls isoflavonoid biosynthesis.

Keywords: Biosynthesis - Hairy root - 2-Hydroxyisoflavanone synthase - 2-Hydroxyisoflavanone dehydratase - Isoflavone - Lotus japonicus

Abbreviations: GSH, reduced glutathione; GUS, β-glucuronidase; IFS, 2-hydroxyisoflavanone synthatase; HI4'OMT, 2-hydroxyisoflavanone 4'-O-methyltransferase; HID, 2-hydroxyisoflavanone dehydratase; LC-FTICR/MS, liquid chromatography-Fourier transform cyclotron resonance/mass spectrometry; RT–PCR, reverse transcription–PCR.

(Received July 18, 2007; Accepted September 28, 2007)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.