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Plant and Cell Physiology Advance Access originally published online on October 15, 2007
Plant and Cell Physiology 2007 48(11):1635-1643; doi:10.1093/pcp/pcm138
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Purification and Characterization of UDP-Glucose : Curcumin Glucoside 1,6-Glucosyltransferase from Catharanthus roseus Cell Suspension Cultures

Yukie Oguchi1, Sayaka Masada1, Toshiya Kondo2, Kazuyoshi Terasaka1 and Hajime Mizukami1,*

1Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, 467-8603 Japan
2School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo, 108-8641 Japan

*Corresponding author: E-mail, hajimem{at}phar.nagoya-cu.ac.jp; Fax, +81-52-836-3415.


  Abstract

Catharanthus roseus cell suspension cultures converted exogenously added curcumin to a series of curcumin glucosides that possessed drastically enhanced water solubility. A cDNA clone encoding a glucosyltransferase responsible for glucosylation of curcumin to form curcumin 4'-O-glucoside was previously isolated, and in the present study a novel sugar–sugar glycosyltransferase, UDP-glucose:curcumin glucoside glucosyltransferase (UCGGT), was purified approximately 900-fold to apparent homogeneity from cultured cells of C. roseus. The purified enzyme (0.2% activity yield) catalyzed 1,6-glucosylation of curcumin 4'-O-glucoside to yield curcumin 4'-O-gentiobioside. The molecular weight and isoelectric point were estimated to be about 50 kDa and 5.2, respectively. The enzyme showed a pH optimum between 7.5 and 7.8. Both flavonoid 3-O- and 7-O-glucosides were also preferred acceptor substrates of the enzyme, whereas little activity was shown toward simple phenolic glucosides such as arbutin and glucovanillin, cyanogenic glucoside (prunasin) or flavonoid galactoside. These results suggest that UCGGT may also function in the biosynthesis of flavonoid glycosides in planta.

Keywords: Catharanthus roseus - Characterization - Curcumin glucoside - 1,6-Glucosyltransferase - Purification

Abbreviations: Cdg, curcumin 4',4''-O-diglucoside; Cmg, curcumin 4'-O-glucoside; Cmgen, curcumin 4'-O-gentiobioside; DMSO, dimethylsulfoxide; GT, glycosyltransferase; HMBC, heteronuclear multibond connectivity; MJ, methyl jasmonate; NMR, nuclear magnetic resonance; UCGGT, UDP-glucose:curcumin glucoside glucosyltransferase; UCGT, UDP-glucose:curcumin glucosyltransferase.

(Received August 31, 2007; Accepted October 10, 2007)
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