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Plant and Cell Physiology Advance Access originally published online on December 5, 2006
Plant and Cell Physiology 2007 48(1):3-7; doi:10.1093/pcp/pcl049
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© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

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Cell Type-Specific Gene Expression Profiling in Plants by Using a Combination of Laser Microdissection and High-Throughput Technologies

Kazuhiro Ohtsu1, Hirokazu Takahashi2, Patrick S. Schnable1,3 and Mikio Nakazono2,*

1Department of Agronomy, Iowa State University, Ames, IA 50011-3650, USA
2Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo, 113-8657 Japan
3Center for Plant Genomics, Iowa State University, Ames, IA 50011-3650, USA

*Corresponding author: E-mail, anakazo{at}mail.ecc.u-tokyo.ac.jp; Fax, +81-3-5841-5183.


  Abstract

Laser microdissection (LM) allows for the isolation of specific cells of interest from heterogeneous tissues under direct microscopic visualization with the assistance of a laser beam. By permitting global analyses of gene expression and metabolites in the selected cells, it is a powerful tool for understanding the biological processes in individual cell types during development or in response to various stimuli. Recently, LM technology has been successfully applied to the separation of individual plant cell types. Here, we provide an overview of applications of LM combined with high-throughput technologies including transcript analyses [microarrays, serial analysis of gene expression (SAGE) and 454-sequencing], proteomic analyses and metabolomic profiling, for cell type-specific gene expression analyses in plants.

Keywords: 454-sequencing - Laser microdissection (LM) - Microarray - Serial analysis of gene expression (SAGE)

Abbreviations: EST, expressed sequence tag; FAS, fluorescence-activated sorting; LM, laser microdissection; SAGE, serial analysis of gene expression; SAM, shoot apical meristem; SNP, single nucleotide polymorphism

(Received September 18, 2006; Accepted November 28, 2006)
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