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Plant and Cell Physiology Advance Access originally published online on December 5, 2006
Plant and Cell Physiology 2007 48(1):193-197; doi:10.1093/pcp/pcl051
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© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

Bowman–Birk Proteinase Inhibitor Confers Heavy Metal and Multiple Drug Tolerance in Yeast

Nobukazu Shitan1, Ken-ichi Horiuchi2, Fumihiko Sato3 and Kazufumi Yazaki1,*

1Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji, 611-0011 Japan
2Materials and Analyses Team, Core Technology Development Division, Technical Center, Nitta Corporation, 172, Ikezawacho, Yamatokoriyama, Nara, 639-1085 Japan
3Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, 606-8502 Japan

*Corresponding author: E-mail, yazaki{at}rish.kyoto-u.ac.jp; Fax, +81-774-38-3623.


  Abstract

Cultured Coptis japonica cells show tolerance to various toxic compounds. By yeast functional screening of cadmium (Cd) plates with its cDNA library, we isolated a gene encoding Bowman–Birk proteinase inhibitor (CjBBI). The yeast transformant of CjBBI showed multiple tolerance to various drugs adding to Cd, and revealed reduced Cd accumulation in cells. Preferential organs for Cjbbi expression were aerial parts of intact plants, and the subcellular localization of CjBBI was shown, using its green fluorescent protein fusion, to be the apoplast. Induction of Cjbbi expression by Cd treatment suggested that CjBBI was responsible for the tolerance to Cd observed in C. japonica cells.

Keywords: Alkaloid - Cadmium - Coptis japonica - Functional screening - Multiple drug tolerance – Proteinase inhibitor

Abbreviations: BBI, Bowman–Birk proteinase inhibitor; Cd, cadmium; GFP, green fluorescent protein; MT, metallothionein; SD, synthetic dextrose

The nucleotide sequences reported in this paper have been submitted to the DDBJ database under accession numbers AB257868 (Cjbbi mRNA) and AB257869 (Cjmt mRNA).

(Received November 9, 2006; Accepted November 27, 2006)
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