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Plant and Cell Physiology Advance Access originally published online on November 21, 2006
Plant and Cell Physiology 2007 48(1):19-30; doi:10.1093/pcp/pcl036
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© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Disruption of Actin Filaments by Latrunculin B Affects Cell Wall Construction in Picea meyeri Pollen Tube by Disturbing Vesicle Trafficking

Tong Chen1,2, Nianjun Teng1,2, Xiaoqin Wu1,2, Yuhua Wang1,2, Wei Tang3, Jozef Samaj4,5, Frantisek Baluska4,6 and Jinxing Lin1,*

1Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China
2Graduate School of the Chinese Academy of Sciences, Beijing 100049, PR China
3Department of Biology, Howell Science Complex, East Carolina University, Greenville, NC 27858-4353, USA
4Institute of Cellular and Molecular Botany, University of Bonn, Kirschallee 1, D-53115 Bonn, Germany
5Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademicka 2, SK-95007 Nitra, Slovak Republic
6Institute of Botany, Slovak Academy of Sciences, Dubravska 14, SK-84223 Bratislava, Slovak Republic

*Corresponding author: E-mail, linjx{at}ibcas.ac.cn; Fax, +86-10-62590833.


  Abstract

The involvement of actin filaments (AFs) in vesicle trafficking, cell wall construction and tip growth was investigated during pollen tube development of Picea meyeri. Pollen germination and tube elongation were inhibited in a dose-dependent manner by the latrunculin B (LatB) treatment. The fine AFs were broken down into disorganized fragments showing a tendency to aggregate. FM4-64 labeling revealed that the dynamic balance of vesicle trafficking was perturbed due to F-actin disruption and the fountain-like cytoplasmic pattern changed into disorganized Brownian movement. The configuration and/or distribution of cell wall components, such as pectins, callose and cellulose, as well as arabinogalactan proteins changed in obvious ways after the LatB application. Fourier transform infrared (FTIR) analysis further established significant changes in the chemical composition of the wall material. Our results indicate that depolymerization of AFs affects the distribution and configuration of cell wall components in Picea meyeri pollen tube by disturbing vesicle trafficking.

Keywords: Actin filaments - Cell wall - Picea meyeri - Pollen tube growth - Vesicle trafficking

Abbreviations: AF, actin filament; DIC, differential interference contrast; DMSO, dimethylsulfoxide; FTIR, Fourier transform infrared; LatB, latrunculin B; LSCM, laser scanning confocal scanning microscopy; TEM, transmission electron microscopy.

(Received October 18, 2006; Accepted November 10, 2006)
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