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Plant and Cell Physiology Advance Access originally published online on December 5, 2006
Plant and Cell Physiology 2007 48(1):134-146; doi:10.1093/pcp/pcl048
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© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Temporary Expression of the TAF10 Gene and its Requirement for Normal Development of Arabidopsis thaliana

Yosuke Tamada1,3, Kazuki Nakamori1,4, Hiromi Nakatani1, Kentaro Matsuda2,5, Shingo Hata1,2, Tsuyoshi Furumoto1,6,* and Katsura Izui1,2,7

1Laboratory of Plant Physiology, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, 606-8502 Japan
2Division of Applied Biosciences, Faculty of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502 Japan

*Corresponding author: E-mail, tfurumoto{at}hiroshima-u.ac.jp; Fax, +81-82-424-7453.


  Abstract

TAF10 is one of the TATA box-binding protein (TBP)-associated factors (TAFs) which constitute a TFIID with a TBP. Initially most TAFs were thought to be necessary for accurate transcription initiation from a broad group of core promoters. However, it was recently revealed that several TAFs are expressed in limited tissues during animal embryogenesis, and are indispensable for normal development of the tissues. They are called ‘selective’ TAFs. In plants, however, little is known as to these ‘selective’ TAFs and their function. Here we isolated the Arabidopsis thaliana TAF10 gene (atTAF10), which is a single gene closely related to the TAF10 genes of other organisms. atTAF10 was expressed transiently during the development of several organs such as lateral roots, rosette leaves and most floral organs. Such an expression pattern was clearly distinct from that of Arabidopsis Rpb1, which encodes a component of RNA polymerase II, suggesting that atTAF10 functions in not only general transcription but also the selective expression of a subset of genes. In a knockdown mutant of atTAF10, we observed several abnormal phenotypes involved in meristem activity and leaf development, suggesting that atTAF10 is concerned in pleiotropic, but selected morphological events in Arabidopsis. These results clearly demonstrate that TAF10 is a ‘selective’ TAF in plants, providing a new insight into the function of TAFs in plants.

Keywords: Arabidopsis thaliana - General transcription factor - Morphogenesis - TAF10 - T-DNA insertion mutant

Abbreviations: atTAF10, Arabidopsis thaliana TAF10; CaMV, cauliflower mosaic virus; dmTAF10, Drosophila melanogaster TAF10; ftTAF10, Flaveria trinervia TAF10; GA3, gibberellin A3; GTF, general transcription factor; GUS, ß-glucuronidase; mmTAF10, Mus musculus TAF10; NAA, {alpha}-naphthalene acetic acid; NPA, N-(1-naphthyl)phthalamic acid; ORF, open reading frame; PIC, pre-initiation complex; pnh, pinhead; RNAP2, RNA polymerase II; SAM, shoot apical meristem; scTAF10, Saccharomyces cerevisiae TAF10; TAF, TATA box-binding protein-associated factor; TBP, TATA box-binding protein; TFIID, transcription factor IID; tfl2, terminal flower2; VM, vegetative meristem; wus, wuschel.

3 Present address: Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706, USA

4 Present address: Yakult Central Institute for Microbiological Research, Yakult Honsha Co., Ltd, Kunitachi, Tokyo, 186-0011 Japan

5 Present address: Laboratory of Science Communication and Bioethics, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, 606-8502 Japan

6 Present address: Department of Biological Science, Graduate School of Science, Hiroshima University, Kagamiyama 1-3-1, Higashi-Hiroshima, 739-8526 Japan

7 Present address: Department of Biotechnological Science, Kinki University, Nishimitani Kinokawa, Wakayama, 649-6493 Japan

(Received July 7, 2006; Accepted November 24, 2006)
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