Plant and Cell Physiology Advance Access originally published online on July 18, 2006
Plant and Cell Physiology 2006 47(8):1058-1068; doi:10.1093/pcp/pcj076
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Ectopic Expression of the Cotton Non-symbiotic Hemoglobin Gene GhHbd1 Triggers Defense Responses and Increases Disease Tolerance in Arabidopsis
1 State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China
2 Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, PR China
3 College of Life Science, Hebei University, Baoding 071002, PR China
4 Graduate School of the Chinese Academy of Sciences, Beijing, PR China
* Corresponding author: E-mail, guixianx{at}yahoo.com; Fax, +861-062548243.
Plant non-symbiotic hemoglobins (nsHbs) play important roles in a variety of cellular processes. Previous evidence from this laboratory indicates that the expression of a class 1 nsHb gene (GhHb1) from cotton is induced in cotton roots challenged with the Verticillium wilt fungus. The present study examined further the expression patterns of the GhHb1 gene in cotton plants and characterized its in vivo function through ectopic overexpression of the gene in Arabidopsis thaliana. Expression of GhHb1 in cotton plants was induced by exogenously applied salicylic acid, methyl jasmonic acid, ethylene, hydrogen peroxide (H2O2) and nitric oxide (NO). Ectopic overproduction of GhHb1 in Arabidopsis led to constitutive expression of the defense genes PR-1 and PDF1.2, and conferred enhanced disease resistance to Pseudomonas syringae and tolerance to V. dahliae. GhHb1-transgenic Arabidopsis seedlings were more tolerant to exogenous NO and contained lower levels of cellular NO than the wild-type control. Moreover, transgenic plants with relatively high levels of expression of the GhHb1 gene developed spontaneous hypersensitive lesions on the leaves in the absence of pathogen inoculation. Our results indicate that GhHb1 proteins play a role in the defense responses against pathogen invasions, possibly by modulating the NO level and the ratio of H2O2/NO in the defense process.
(Received April 12, 2006; Accepted June 5, 2006)
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Jokipii-Lukkari, A. D. Frey, P. T. Kallio, and H. Haggman Intrinsic non-symbiotic and truncated haemoglobins and heterologous Vitreoscilla haemoglobin expression in plants J. Exp. Bot., February 1, 2009; 60(2): 409 - 422. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zhang and D. Xing Methyl Jasmonate Induces Production of Reactive Oxygen Species and Alterations in Mitochondrial Dynamics that Precede Photosynthetic Dysfunction and Subsequent Cell Death Plant Cell Physiol., July 1, 2008; 49(7): 1092 - 1111. [Abstract] [Full Text] [PDF]
|
|