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Plant and Cell Physiology Advance Access originally published online on May 13, 2006
Plant and Cell Physiology 2006 47(7):829-838; doi:10.1093/pcp/pcj054
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© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Identification of a Sed5-like SNARE Gene LjSYP32-1 that Contributes to Nodule Tissue Formation of Lotus japonicus

Ha Thu Mai1,3, Mika Nomura1, Kaoru Takegawa1, Erika Asamizu2, Syusei Sato2, Tomohiko Kato2, Satoshi Tabata2 and Shigeyuki Tajima1,*

1 Department of Life Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa, 761-0795 Japan
2 Kazusa DNA Research Institute, 1532-3 Yana, Kisarazu, Chiba, 292-0812 Japan
3 Institute of Agricultural Genetics, Conhue-Tuliem, Hanoi, Vietnam

* Corresponding author: E-mail, tajima{at}ag.kagawa-u.ac.jp; Fax, +81-87-891-3021.

We identified a Sed5-like clone LjSYP32-1 which contributes to nodule tissue formation and plant growth in Lotus japonicus. In the L. japonicus expressed sequence tag (EST) clone databases of Kazusa DNA Research Institute, another syntaxin-related clone (LjSYP32-2) was also detected, and the nucleotide and amino acid sequences of these two clone are very similar to each other. Real-time PCR and promoter analysis indicated that expression of LjSYP32-1 was dominant compared with LjSYP32-2 in the various plant organs. Promoter analysis and in situ hybridization revealed that LjSYP32-1 was expressed significantly in the inner cortex cell layer surrounding the infected zone of young nodules and in the meristem area of developing lateral root. To explore the function and physiological role of LjSYP32-1 in nodules and other plant organs, stable transformation lines of L. japonicus expressing either sense or antisense LjSYP32-1 were prepared. The antisense plants showed a significantly retarded plant growth phenotype, suggesting a role for LjSYP32-1 in supporting plant growth. In the same transgenic lines, the plants were capable of forming nodules, but the acetylene reduction activity was reduced by around 50% per plant. The nodules were much smaller and some nodules were fused to each other by sharing the inner cortex. The rate of occurence of such irregular nodules was twice that observed in wild-type plants. The data suggest that LjSYP32-1 contributes to the support of plant growth and normal nodule tissue differentiation.

(Received January 21, 2006; Accepted April 28, 2006)
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