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Plant and Cell Physiology Advance Access originally published online on April 11, 2006
Plant and Cell Physiology 2006 47(6):736-742; doi:10.1093/pcp/pcj045
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Increase of Homologous Recombination Frequency in Vascular Tissue of Arabidopsis Plants Exposed to Salt Stress

Alex Boyko, Darryl Hudson1, Prasanna Bhomkar, Palak Kathiria and Igor Kovalchuk*

Department of Biological Sciences, University of Lethbridge, Lethbridge, AB, T1K 3M4, Canada

* Corresponding author: E-mail, igor.kovalchuk{at}uleth.ca; Fax, +1-403 329 2242.

Here we analyzed the influence of salt stress on plant genome stability. Homologous recombination events were detected in transgenic Arabidopsis plants that carried in their genome a ß-glucuronidase recombination marker. Recombination events were scored as blue sectors using a stereo microscope. Exposure to 50 mM salt resulted in a 3.0-fold increase in recombination frequency. To analyze the organ and tissue specificity of recombination events, we examined cross-sections of leaves, stems and roots. We found that nearly 30% of recombination events in plants grown under normal conditions and nearly 50% of events in plants grown on salt were undetected by the conventional method. Most of the recombination events represented a cluster/group of cells (12 on average), although events with single cells were also detected. Recombination events were very frequent in leaf mesophyll cells. On average, individual recombination events located on leaves contained more cells than events located on roots or stems. Analysis of recombination events in cross-sectioned tissue of salt-treated plants revealed a shift in the distribution of recombination events towards the vascular tissue. We discuss the significance of the finding for plant stress physiology.

1 Present address: Department of Molecular and Cellular Biology (MCB), University of Guelph, Axlerod building, Guelph, Ontario, N1G 2W1, Canada

(Received January 20, 2006; Accepted March 31, 2006)
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