Plant and Cell Physiology Advance Access originally published online on March 13, 2006
Plant and Cell Physiology 2006 47(5):653-663; doi:10.1093/pcp/pcj034
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
The Wound Response Mutant suppressor of prosystemin-mediated responses6 (spr6) is a Weak Allele of the Tomato Homolog of CORONATINE-INSENSITIVE1 (COI1)
1 State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, PR China
2 Graduate School of Chinese Academy of Sciences, Beijing 100039, PR China
3 The Key Laboratory of Crop Biology, Agronomy College, Shandong Agricultural University, Taian 271018, PR China
4 Institute of Applied Entomology, Zhejiang University, Hangzhou 310029, PR China
* Corresponding author: E-mail, cyli{at}genetics.ac.cn; Fax, +8610-64873428.
The systemic defense response of tomato plant in response to insect attack and wounding is regulated by the 18 amino acid peptide systemin and the phytohormone jasmonic acid (JA). Recent genetic analyses based mainly on spr (suppressors of prosystemin-mediated responses) mutant screens have led to the hypothesis that systemin acts at, or near, the site of wounding to amplify the production of JA, which in turn functions as a mobile signal to promote the systemic defense response. In order to identify more components involved in the systemin/JA-signaled defense response, we carried out a larger scale screen for new spr mutants in tomato. Here we describe the characterization of spr6, a mutant impaired in wound- and systemin-induced defense gene expression. Using a candidate gene approach based on genetic linkage, we demonstrate that spr6 is allelic to jai1-1, which is a loss-of-function allele of the tomato homolog of CORONATINE-INSENSITIVE1 (COI1), an F-box protein that is required for JA-signaled processes in Arabidopsis. We show several aspects of the spr6 mutant phenotype distinct from that of jai1-1. First, the responsiveness of spr6 plants to exogenous JA shows a dosage dependency, i.e. it is more sensitive to JA than jai1-1 while less sensitive to JA than the wild-type. Secondly, unlike the sterile jai1-1, the spr6 plant displays normal fertility and seed set and thus can be maintained as a pure line and does not require selection. Therefore, spr6 provides a valuable tool, which can complement the limitations of jai1-1, to study JA signaling in tomato. The gene identification process of Spr6 we described herein represents an example showing the convenience of a candidate gene approach, based on genetic linkage, to identify gene functions of genetic loci defined by tomato wound response mutants.
5 These authors contributed equally to this work.
(Received December 25, 2005; Accepted March 9, 2006)
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Shoji, T. Ogawa, and T. Hashimoto Jasmonate-Induced Nicotine Formation in Tobacco is Mediated by Tobacco COI1 and JAZ Genes Plant Cell Physiol., July 1, 2008; 49(7): 1003 - 1012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Zhai, C.-B. Li, W. Zheng, X. Wu, J. Zhao, G. Zhou, H. Jiang, J. Sun, Y. Lou, and C. Li Phytochrome Chromophore Deficiency Leads to Overproduction of Jasmonic Acid and Elevated Expression of Jasmonate-Responsive Genes in Arabidopsis Plant Cell Physiol., July 1, 2007; 48(7): 1061 - 1071. [Abstract] [Full Text] [PDF]
|
|