Plant and Cell Physiology Advance Access originally published online on March 15, 2006
Plant and Cell Physiology 2006 47(5):572-580; doi:10.1093/pcp/pcj035
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Rapid Paper |
Arabidopsis UPF1 RNA Helicase for Nonsense-mediated mRNA Decay is Involved in Seed Size Control and is Essential for Growth
Laboratory of Biochemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601 Japan
1 Corresponding author: E-mail, kenzo{at}agr.nagoya-u.ac.jp; Fax, +81-52-789-4094.
UPF1 RNA helicase plays a central role in nonsense-mediated mRNA decay (NMD), which specifically recognizes aberrant mRNAs containing premature termination codons and targets them for degradation. Although NMD factors are highly conserved among eukaryotes, little is known about the role of NMD in plant growth and development. The lba1 mutant of Arabidopsis thaliana with a Gly851
Glu missense mutation in AtUPF1 yielded seeds that were on average 22% longer in the long axis and 35% heavier than the wild-type Col seeds. Expression of the wild-type AtUPF1 in this mutant reduced the seeds to a normal size. During early stages of seed development, globular to torpedo stages of the embryos were contained within seed sacs that were larger in lba1 than in Col. Furthermore, the distance between seeds in siliques was greater in lba1 than in Col, suggesting that the lba1 mutation may affect ovule development. Self-pollinated atupf1-3(+/–) plants heterozygous for AtUPF1 disrupted by T-DNA insertion developed atupf1-3(–/–) seeds with a size and shape similar to those of Col seeds. However, the atupf1-3(–/–) seedlings stopped growing after radicle emergence from the seed coat, and this seedling lethality was rescued by expressing the wild-type AtUPF1. These results suggest that the lba1 mutation in AtUPF1 maternally affects seed development and that AtUPF1 is essential for seedling growth.
(Received February 17, 2006; Accepted March 9, 2006)
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  N. Wittkopp, E. Huntzinger, C. Weiler, J. Sauliere, S. Schmidt, M. Sonawane, and E. Izaurralde Nonsense-Mediated mRNA Decay Effectors Are Essential for Zebrafish Embryonic Development and Survival Mol. Cell. Biol., July 1, 2009; 29(13): 3517 - 3528. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Kurihara, A. Matsui, K. Hanada, M. Kawashima, J. Ishida, T. Morosawa, M. Tanaka, E. Kaminuma, Y. Mochizuki, A. Matsushima, et al. Genome-wide suppression of aberrant mRNA-like noncoding RNAs by NMD in Arabidopsis PNAS, February 17, 2009; 106(7): 2453 - 2458. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Jiao, J. L. Riechmann, and E. M. Meyerowitz Transcriptome-Wide Analysis of Uncapped mRNAs in Arabidopsis Reveals Regulation of mRNA Degradation PLANT CELL, October 1, 2008; 20(10): 2571 - 2585. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Laubinger, T. Sachsenberg, G. Zeller, W. Busch, J. U. Lohmann, G. Ratsch, and D. Weigel Dual roles of the nuclear cap-binding complex and SERRATE in pre-mRNA splicing and microRNA processing in Arabidopsis thaliana PNAS, June 24, 2008; 105(25): 8795 - 8800. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Li, H. Liu, H. Zhang, X. Wang, and F. Song OsBIRH1, a DEAD-box RNA helicase with functions in modulating defence responses against pathogen infection and oxidative stress J. Exp. Bot., May 1, 2008; 59(8): 2133 - 2146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Aki, M. Konishi, T. Kikuchi, T. Fujimori, T. Yoneyama, and S. Yanagisawa Distinct modulations of the hexokinase1-mediated glucose response and hexokinase1-independent processes by HYS1/CPR5 in Arabidopsis J. Exp. Bot., September 6, 2007; (2007) erm169v2. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Johns, A. Grimson, S. L. Kuchma, C. L. Newman, and P. Anderson Caenorhabditis elegans SMG-2 Selectively Marks mRNAs Containing Premature Translation Termination Codons Mol. Cell. Biol., August 15, 2007; 27(16): 5630 - 5638. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Hori and Y. Watanabe Context Analysis of Termination Codons in mRNA that are Recognized by Plant NMD Plant Cell Physiol., July 1, 2007; 48(7): 1072 - 1078. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kertesz, Z. Kerenyi, Z. Merai, I. Bartos, T. Palfy, E. Barta, and D. Silhavy Both introns and long 3'-UTRs operate as cis-acting elements to trigger nonsense-mediated decay in plants Nucleic Acids Res., December 4, 2006; 34(21): 6147 - 6157. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ner-Gaon and R. Fluhr Whole-Genome Microarray in Arabidopsis Facilitates Global Analysis of Retained Introns DNA Res, January 1, 2006; 13(3): 111 - 121. [Abstract] [Full Text] [PDF]
|
|