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Plant and Cell Physiology Advance Access originally published online on December 28, 2005
Plant and Cell Physiology 2006 47(3):426-431; doi:10.1093/pcp/pci251
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Short Communication

A Highly Efficient Transformation Protocol for Micro-Tom, a Model Cultivar for Tomato Functional Genomics

Hyeon-Jin Sun1, Sayaka Uchii1, Shin Watanabe and Hiroshi Ezura*

Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8572 Japan

* Corresponding author: E-mail, ezura{at}gene.tsukuba.ac.jp; Fax, +81-29-853-7263.

We report a highly efficient protocol for the Agrobacterium-mediated genetic transformation of a miniature dwarf tomato (Lycopersicon esculentum), Micro-Tom, a model cultivar for tomato functional genomics. Cotyledon explants of tomato inoculated with Agrobacterium tumefaciens (Rhizobium radiobacter) C58C1RifR harboring the binary vector pIG121Hm generated a mass of chimeric non-transgenic and transgenic adventitious buds. Repeated shoot elongation from the mass of adventitious buds on selection media resulted in the production of multiple transgenic plants that originated from independent transformation events. The transformation efficiency exceeded 40% of the explants. This protocol could become a powerful tool for functional genomics in tomato.

1 These authors contributed equally to this work.

(Received November 16, 2005; Accepted December 23, 2005)
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