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Plant and Cell Physiology Advance Access originally published online on January 17, 2006
Plant and Cell Physiology 2006 47(3):419-425; doi:10.1093/pcp/pcj009
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Action Spectrum for Expression of the High Intensity Light-inducible Lhc-like Gene Lhl4 in the Green Alga Chlamydomonas reinhardtii

Haruhiko Teramoto1,2,5,*, Asako Ishii1, Yukihiro Kimura1,2, Koji Hasegawa1,2, Shigeaki Nakazawa1, Takanori Nakamura3, Sho-ichi Higashi3, Masakatsu Watanabe3,4 and Taka-aki Ono1,2,*

1 Laboratory for Photo-Biology (1), Photodynamics Research Center, RIKEN, Sendai, 980-0845 Japan
2 Molecular Membrane Laboratory, RIKEN, 2-1 Hirosawa, Saitama, 351-0198 Japan
3 National Institute for Basic Biology, Myodaiji, Okazaki, Aichi, 444-8585 Japan
4 Department of Photoscience, School of Advanced Science, Graduate University for Advanced Studies, Shonan Village, Hayama, Kanagawa, 240-0193 Japan

* Corresponding authors: Takaki Ono, E-mail, takaaki{at}riken.jp; Fax, +81-48-462-1330; Haruhiko Teramoto, E-mail, teramoto{at}rite.or.jp.

Lhl4 encodes a distant relative of light-harvesting Chl-a/b proteins in the green alga Chlamydomonas reinhardtii. Lhl4 mRNA markedly accumulated within 30 min after illumination and in proportion to the light intensity up to a fluence rate much higher than that required for photosynthesis. The high intensity light (HL)-induced accumulation of Lhl4 mRNA required continuous illumination, and the mRNA level rapidly decreased when the cells were placed in the dark. HL only slightly stabilized the mRNA, suggesting that the HL-induced expression of the Lhl4 gene is primarily regulated at the level of transcription. Blue light was more effective for inducing Lhl4 gene expression than green or red light, and far-red light had no effect. The action spectrum for Lhl4 gene expression was examined at wavelengths between 325 and 775 nm using the Okazaki Large Spectrograph. The obtained spectrum showed a distinct peak in the blue region (450 nm) and a shoulder in the UV-A region (375 nm). The curve in the spectrum rose steeply in the short wavelength UV region. In addition, we observed two minor peaks in the green (575 nm) and the red (675 nm) regions. The action spectrum suggests that a blue/UV-A light photoreceptor with a flavin-based chromophore participates in the HL response of Lhl4 gene expression. However, the hypersensitivity to near UV-B light suggests the involvement of an unidentified UV light perception system in the expression of the Lhl4 gene.

5 Present address: Research Institute of Innovative Technology for the Earth (RITE), 9-2 Kizugawadai, Kizu-cho Soraku-gun, Kyoto, 619-0292 Japan.

(Received October 17, 2005; Accepted January 9, 2006)
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