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Plant and Cell Physiology Advance Access originally published online on November 11, 2006
Plant and Cell Physiology 2006 47(12):1683-1686; doi:10.1093/pcp/pcl034
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© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

Expression of B2-Type Cyclin-Dependent Kinase is Controlled by Protein Degradation in Arabidopsis thaliana

Sumiko Adachi1,2, Hirofumi Uchimiya2 and Masaaki Umeda1,*

1 Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama 8916-5, Ikoma, Nara, 630-0101 Japan
2 Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-0032 Japan

* Corresponding author: E-mail, mumeda{at}bs.naist.jp; Fax, +81-443-72-5599.


  Abstract

The eukaryotic cell cycle is controlled by cyclin-dependent kinases (CDKs). Plants possess six types of CDK, among which the B-type CDK (CDKB) is expressed specifically from the late S- to the M-phase. We demonstrate that the expression of Arabidopsis CDKB2 is under the control of the protein degradation machinery. ß-Glucuronidase fused to a putative PEST motif of CDKB2 was unstable in tobacco Bright Yellow-2 cells and Arabidopsis plants, and its degradation was arrested by the proteasome inhibitor MG132. We propose that the abundance of CDKB2 protein is regulated not only at the transcriptional level, but also through proteasome-mediated protein degradation.

Keywords: Arabidopsis thaliana - Cyclin-dependent kinase - PEST motif - Protein degradation

Abbreviations: BY-2, Bright Yellow-2; CDK, cyclin-dependent kinase; CDKA, A-type CDK; CDKB, B-type CDK; CHX, cycloheximide; DMSO, dimethylsulfoxide; GUS, ß-glucuronidase; RT–PCR, reverse transcription–PCR.

(Received October 5, 2006; Accepted November 8, 2006)
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