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Plant and Cell Physiology Advance Access originally published online on November 10, 2006
Plant and Cell Physiology 2006 47(12):1663-1673; doi:10.1093/pcp/pcl033
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© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Proteome Analysis of Bell Pepper (Capsicum annuum L.) Chromoplasts

Muhammad Asim Siddique, Jonas Grossmann, Wilhelm Gruissem and Sacha Baginsky*

Institute of Plant Sciences, ETH Zürich, Universitätstrasse 2, CH-8092 Zürich, Switzerland

* Corresponding author: E-mail, sbaginsky{at}ethz.ch; Fax, +41-1-632-10-79.


  Abstract

We report a comprehensive proteome analysis of chromoplasts from bell pepper (Capsicum annuum L.). The combination of a novel strategy for database-independent detection of proteins from tandem mass spectrometry (MS/MS) data with standard database searches allowed us to identify 151 proteins with a high level of confidence. These include several well-known plastid proteins but also novel proteins that were not previously reported from other plastid proteome studies. The majority of the identified proteins are active in plastid carbohydrate and amino acid metabolism. Among the most abundant individual proteins are capsanthin/capsorubin synthase and fibrillin, which are involved in the synthesis and storage of carotenoids that accumulate to high levels in chromoplasts. The relative abundances of the identified chromoplast proteins differ remarkably compared with their abundances in other plastid types, suggesting a chromoplast-specific metabolic network. Our results provide an overview of the major metabolic pathways active in chromoplasts and extend existing knowledge about prevalent metabolic activities of different plastid types.

Keywords: Biosynthesis - Carotenoid - Chromoplast - Mass spectrometry - Metabolism - Proteomics

Abbreviations: CCS, capsanthin/capsorubin synthase; DTT, dithiothreitol; MS/MS, tandem mass spectrometry; NSI, nanospray ionization; OPPP, oxidative branch of the pentose phosphate pathway; RP-LC, reverse phase liquid chromatography; SQD1, UDP sulfoquinovose synthase.

(Received October 16, 2006; Accepted November 8, 2006)
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