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Plant and Cell Physiology Advance Access originally published online on October 3, 2006
Plant and Cell Physiology 2006 47(11):1582-1589; doi:10.1093/pcp/pcl018
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© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

Genetic Transformation of Populus trichocarpa Genotype Nisqually-1: A Functional Genomic Tool for Woody Plants

Jingyuan Song1,2, Shanfa Lu2, Zenn-Zong Chen3, Rodrigo Lourenco2 and Vincent L. Chiang2,*

1Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Xibeiwang, Haidian District, Beijing 100094, PR China
2Forest Biotechnology Group, Department of Forestry and Environmental Resources, College of Natural Resources, North Carolina State University, Raleigh, NC 27695, USA
3Division of Silviculture, Taiwan Forestry Research Institute, 53 Nan-Hai Road, Taipei 100, Taiwan

* Corresponding author: E-mail, vincent_chiang{at}ncsu.edu; Fax, +1-919-515-7801.


  Abstract

We report here the Agrobacterium-mediated genetic transformation of Nisqually-1, a Populus trichocarpa genotype whose genome was recently sequenced. Several systems were established. Internodal stem segments from vigorously growing greenhouse plants are the explants most amenable to transformation. For the most efficient system, approximately 40% of the stem segments infected with pBI121-containing Agrobacterium tumefaciens C58 produced transgenic calli, as confirmed by ß-glucuronidase (GUS) staining. The regeneration efficiency of independent transgenic plants was approximately 13%, as revealed by genomic Southern analysis. Some transgenic plants were produced in as little as 5 months after co-cultivation. This system may help to facilitate studies of gene functions in tree growth and development at a genome level.

Keywords: Agrobacterium tumefaciens - Black cottonwood - Functional genomics - Genetic transformation - Nisqually-1 - Populus trichocarpa

Abbreviations: 6-BA, 6-benzyladenine; CIM, callus induction medium; GUS, ß-glucuronidase; IBA, indole-3-butyric acid; MS, Murashige and Skoog; NAA, 1-naphthylacetic acid; nptII, neomycin phosphotransferase II gene; RM, rooting medium; SIM, shoot induction medium; TDZ, thidiazuron (N-phenyl-N'-1,2,3-thiadiazol-5-yl-urea); uidA, ß-glucuronidase gene; WPM, woody plant medium.

(Received August 23, 2006; Accepted September 28, 2006)
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