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Plant and Cell Physiology Advance Access originally published online on August 30, 2006
Plant and Cell Physiology 2006 47(10):1412-1419; doi:10.1093/pcp/pcl003
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© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

Differential Expression of Two Flavonoid 3'-Hydroxylase cDNAs Involved in Biosynthesis of Anthocyanin Pigments and 3-Deoxyanthocyanidin Phytoalexins in Sorghum

Chun-Hat Shih1,2, Ivan K. Chu2,3, Wing Kin Yip1 and Clive Lo1,*

1 Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China
2 Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China
3 Genome Research Centre, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China

* Corresponding author: E-mail, clivelo{at}hkucc.hku.hk; Fax, +852-2858-3477.

Three unique sorghum flavonoid 3'-hydroxylase (F3'H) cDNAs (SbF3'H1, SbF3'H2 and SbF3'H3) were discovered through bioinformatics analysis. Their encoded proteins showed >60% identity to the Arabidopsis TT7 (F3'H) protein. Overexpression of SbF3'H1 or SbF3'H2 restored the ability of tt7 mutants to produce 3'-hydroxylaed flavonoids, establishing their roles as functional F3'H enzymes. In sorghum mesocotyls, SbF3'H1 expression was involved in light-specific anthocyanin accumulation while SbF3'H2 expression was involved in pathogen-specific 3-deoxyanthocyanidin synthesis. No SbF3'H3 expression was detected in all tissues examined. The sorghum mesocotyls represent a good system for investigation of differential regulation of F3'H genes/alleles responding to different external stimuli.

The nucleotide sequences reported in this paper have been submitted to GenBank under accession numbers DQ787855–7 (SbF3'H1-SbF3'H3).

(Received August 1, 2006; Accepted August 16, 2006)
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