Skip Navigation


Plant and Cell Physiology Advance Access originally published online on October 25, 2005
Plant and Cell Physiology 2006 47(1):32-42; doi:10.1093/pcp/pci220
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
47/1/32    most recent
pci220v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (7)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Nakamura, A.
Right arrow Articles by Tanaka, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nakamura, A.
Right arrow Articles by Tanaka, Y.
Agricola
Right arrow Articles by Nakamura, A.
Right arrow Articles by Tanaka, Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


Molecular Cloning, Functional Expression and Subcellular Localization of Two Putative Vacuolar Voltage-gated Chloride Channels in Rice (Oryza sativa L.)

Atsuko Nakamura1,2,*, Atsunori Fukuda2, Shingo Sakai1 and Yoshiyuki Tanaka2

1 Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki, 305-8572 Japan
2 National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305-8602 Japan

* Corresponding author: E-mail, anakamur{at}nias.affrc.go.jp; Fax, +81-298-38-8347.

We isolated two cDNA clones (OsCLC-1 and OsCLC-2) homologous to tobacco CLC-Nt1, which encodes a voltage-gated chloride channel, from rice (Oryza sativa L. ssp. japonica, cv. Nipponbare). The deduced amino acid sequences were highly conserved (87.9% identity with each other). Southern blot analysis of the rice genomic DNA revealed that OsCLC-1 and OsCLC-2 were single-copy genes on chromosomes 4 and 2, respectively. OsCLC-1 was expressed in most tissues, whereas OsCLC-2 was expressed only in the roots, nodes, internodes and leaf sheaths. The level of expression of OsCLC-1, but not of OsCLC-2, was increased by treatment with NaCl. Both genes could partly substitute for GEF1, which encodes the sole chloride channel in yeast, by restoring growth under ionic stress. These results indicate that both genes are chloride channel genes. The proteins from both genes were immunochemically detected in the tonoplast fraction. Tagged synthetic green fluorescent protein which was fused to OsCLC-1 or OsCLC-2 localized in the vacuolar membranes. These results indicate that the proteins may play a role in the transport of chloride ions across the vacuolar membrane. We isolated loss-of-function mutants of both genes from a panel of rice mutants produced by the insertion of a retrotransposon, Tos17, in the exon region, and found inhibition of growth at all life stages.

The nucleotide sequences reported in this paper have been submitted to the DDBJ/EMBL/GenBank database under the accession numbers AB069966 [GenBank] (OsCLC-1) and AB069967 [GenBank] (OsCLC-2).

(Received October 4, 2005; Accepted October 18, 2005)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Phil Trans R Soc BHome page
A. De Angeli, D. Monachello, G. Ephritikhine, J.-M. Frachisse, S. Thomine, F. Gambale, and H. Barbier-Brygoo
CLC-mediated anion transport in plant cells
Phil Trans R Soc B, January 27, 2009; 364(1514): 195 - 201.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Obata, H. K. Kitamoto, A. Nakamura, A. Fukuda, and Y. Tanaka
Rice Shaker Potassium Channel OsKAT1 Confers Tolerance to Salinity Stress on Yeast and Rice Cells
Plant Physiology, August 1, 2007; 144(4): 1978 - 1985.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. J. Miller, X. Fan, M. Orsel, S. J. Smith, and D. M. Wells
Nitrate transport and signalling
J. Exp. Bot., July 1, 2007; 58(9): 2297 - 2306.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Martinoia, M. Maeshima, and H. E. Neuhaus
Vacuolar transporters and their essential role in plant metabolism
J. Exp. Bot., January 1, 2007; 58(1): 83 - 102.
[Abstract] [Full Text] [PDF]

Home page
 Integr. Comp. Biol.Home page
G. A. Pearson and E. A. Serrao
Revisiting synchronous gamete release by fucoid algae in the intertidal zone: fertilization success and beyond?
Integr. Comp. Biol., October 1, 2006; 46(5): 587 - 597.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Endler, S. Meyer, S. Schelbert, T. Schneider, W. Weschke, S. W. Peters, F. Keller, S. Baginsky, E. Martinoia, and U. G. Schmidt
Identification of a Vacuolar Sucrose Transporter in Barley and Arabidopsis Mesophyll Cells by a Tonoplast Proteomic Approach
Plant Physiology, May 1, 2006; 141(1): 196 - 207.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.