Plant and Cell Physiology Advance Access originally published online on November 12, 2005
Plant and Cell Physiology 2006 47(1):141-153; doi:10.1093/pcp/pci230
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Functional Analysis of Rice DREB1/CBF-type Transcription Factors Involved in Cold-responsive Gene Expression in Transgenic Rice
1 Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki, 305-8686 Japan
2 Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, Tsukuba, Ibaraki, 305-0074 Japan
3 Experimental Plant Division, BioResource Center, RIKEN Tsukuba Institute, Tsukuba, Ibaraki, 305-0074 Japan
4 Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, Kawaguchi, Saitama, 332-0012 Japan
5 Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657 Japan
* Corresponding author: E-mail, kazukoys{at}jircas.affrc.go.jp; Fax, +81-29-838-6643.
The transcription factors dehydration-responsive element-binding protein 1s (DREB1s)/C-repeat-binding factors (CBFs) specifically interact with the DRE/CRT cis-acting element and control the expression of many stress-inducible genes in Arabidopsis. The genes for DREB1 orthologs, OsDREB1A and OsDREB1B from rice, are induced by cold stress, and overexpression of DREB1 or OsDREB1 induced strong expression of stress-responsive genes in transgenic Arabidopsis plants, resulting in increased tolerance to high-salt and freezing stresses. In this study, we generated transgenic rice plants overexpressing the OsDREB1 or DREB1 genes. These transgenic rice plants showed not only growth retardation under normal growth conditions but also improved tolerance to drought, high-salt and low-temperature stresses like the transgenic Arabidopsis plants overexpressing OsDREB1 or DREB1. We also detected elevated contents of osmoprotectants such as free proline and various soluble sugars in the transgenic rice as in the transgenic Arabidopsis plants. We identified target stress-inducible genes of OsDREB1A in the transgenic rice using microarray and RNA gel blot analyses. These genes encode proteins that are thought to function in stress tolerance in the plants. These results indicate that the DREB1/CBF cold-responsive pathway is conserved in rice and the DREB1-type genes are quite useful for improvement of stress tolerance to environmental stresses in various kinds of transgenic plants including rice.
6 Present address: Department of Bio-Science, Tokyo University of Agriculture, Setagaya, Tokyo, 156-8502 Japan
7 Present address: RIKEN Plant Science Center, Tsurumi, Yokohama, 203-0045 Japan
(Received May 30, 2005; Accepted November 7, 2005)
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S.-J. Oh, Y. S. Kim, C.-W. Kwon, H. K. Park, J. S. Jeong, and J.-K. Kim Overexpression of the Transcription Factor AP37 in Rice Improves Grain Yield under Drought Conditions Plant Physiology, July 1, 2009; 150(3): 1368 - 1379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Lindlof, M. Brautigam, A. Chawade, O. Olsson, and B. Olsson In silico analysis of promoter regions from cold-induced genes in rice (Oryza sativa L.) and Arabidopsis thaliana reveals the importance of combinatorial control Bioinformatics, June 1, 2009; 25(11): 1345 - 1348. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Ma, X. Dai, Y. Xu, J. Guo, Y. Liu, N. Chen, J. Xiao, D. Zhang, Z. Xu, X. Zhang, et al. Enhanced Tolerance to Chilling Stress in OsMYB3R-2 Transgenic Rice Is Mediated by Alteration in Cell Cycle and Ectopic Expression of Stress Genes Plant Physiology, May 1, 2009; 150(1): 244 - 256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Nakamichi, M. Kusano, A. Fukushima, M. Kita, S. Ito, T. Yamashino, K. Saito, H. Sakakibara, and T. Mizuno Transcript Profiling of an Arabidopsis PSEUDO RESPONSE REGULATOR Arrhythmic Triple Mutant Reveals a Role for the Circadian Clock in Cold Stress Response Plant Cell Physiol., March 1, 2009; 50(3): 447 - 462. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. J. M. Saibo, T. Lourenco, and M. M. Oliveira Transcription factors and regulation of photosynthetic and related metabolism under environmental stresses Ann. Bot., February 1, 2009; 103(4): 609 - 623. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B.-Z. Xiao, X. Chen, C.-B. Xiang, N. Tang, Q.-F. Zhang, and L.-Z. Xiong Evaluation of Seven Function-Known Candidate Genes for their Effects on Improving Drought Resistance of Transgenic Rice under Field Conditions Mol Plant, January 1, 2009; 2(1): 73 - 83. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nakashima, Y. Ito, and K. Yamaguchi-Shinozaki Transcriptional Regulatory Networks in Response to Abiotic Stresses in Arabidopsis and Grasses Plant Physiology, January 1, 2009; 149(1): 88 - 95. [Full Text] [PDF]
|
|
|
|
|
|
Y. Xiang, Y. Huang, and L. Xiong Characterization of Stress-Responsive CIPK Genes in Rice for Stress Tolerance Improvement Plant Physiology, July 1, 2007; 144(3): 1416 - 1428. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Shinozaki and K. Yamaguchi-Shinozaki Gene networks involved in drought stress response and tolerance J. Exp. Bot., January 1, 2007; 58(2): 221 - 227. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. R. Swindell The Association Among Gene Expression Responses to Nine Abiotic Stress Treatments in Arabidopsis thaliana Genetics, December 1, 2006; 174(4): 1811 - 1824. [Abstract] [Full Text] [PDF]
|
|