Plant and Cell Physiology Advance Access originally published online on July 20, 2005
Plant and Cell Physiology 2005 46(9):1568-1577; doi:10.1093/pcp/pci172
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Identification of 33 Rice Aquaporin Genes and Analysis of Their Expression and Function
1 CO2 and Temperature Research Laboratory, National Agricultural Research Center for Tohoku Region, Morioka, 020-0198 Japan
2 Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan
3 United Graduate School of Agricultural Sciences and Cryobiosystem Research Center, Faculty of Agriculture, Iwate University, Morioka, 020-8550 Japan
* Corresponding author: E-mail, junkoi{at}affrc.go.jp; Fax, +81-19-641-7794.
Plant aquaporins form a large protein family including plasma membrane-type (PIPs) and tonoplast-type aquaporins (TIPs), and facilitate osmotic water transport across membranes as a key physiological function. We identified 33 genes for aquaporins in the genome sequence of rice (Oryza sativa L. cv. Nipponbare). We investigated their expression levels in leaf blades, roots and anthers of rice (cv. Akitakomachi) using semi-quantitative reverse transcription–PCR (RT–PCR). At both early tillering (21 d after germination) and panicle formation (56 d) stages, six genes, including OsPIP2;4 and OsPIP2;5, were expressed predominantly in roots, while 14 genes, including OsPIP2;7 and OsTIP1;2, were found in leaf blades. Eight genes, such as OsPIP1;1 and OsTIP4;1, were evenly expressed in leaf blades, roots and anthers. Analysis by stopped-flow spectrophotometry revealed high water channel activity when OsPIP2;4 or OsPIP2;5 were expressed in yeast but not when OsPIP1;1 or OsPIP1;2 were expressed. Furthermore, the mRNA levels of OsPIP2;4 and OsPIP2;5 showed a clear diurnal fluctuation in roots; they showed a peak 3 h after the onset of light and dropped to a minimum 3 h after the onset of darkness. The mRNA levels of 10 genes including OsPIP2;4 and OsPIP2;5 markedly decreased in roots during chilling treatment and recovered after warming. The changes in mRNA levels during and after the chilling treatment were comparable with that of the bleeding sap volume. These results suggested the relationship between the root water uptake and mRNA levels of several aquaporins with high water channel activity, such as OsPIP2;4 and OsPIP2;5.
(Received June 19, 2005; Accepted July 12, 2005)
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  R. B. Heinen, Q. Ye, and F. Chaumont Role of aquaporins in leaf physiology J. Exp. Bot., June 19, 2009; (2009) erp171v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Azad, Y. Sawa, T. Ishikawa, and H. Shibata Heterologous Expression of Tulip Petal Plasma Membrane Aquaporins in Pichia pastoris for Water Channel Analysis Appl. Envir. Microbiol., May 1, 2009; 75(9): 2792 - 2797. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Matsumoto, H.-L. Lian, W.-A. Su, D. Tanaka, C. w. Liu, I. Iwasaki, and Y. Kitagawa Role of the Aquaporin PIP1 Subfamily in the Chilling Tolerance of Rice Plant Cell Physiol., February 1, 2009; 50(2): 216 - 229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.-W. Li, M.-H. Zhang, W.-M. Cai, W.-N. Sun, and W.-A. Su Characterization of OsPIP2;7, a Water Channel Protein in Rice Plant Cell Physiol., December 1, 2008; 49(12): 1851 - 1858. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Murai-Hatano, T. Kuwagata, J. Sakurai, H. Nonami, A. Ahamed, K. Nagasuga, T. Matsunami, K. Fukushi, M. Maeshima, and M. Okada Effect of Low Root Temperature on Hydraulic Conductivity of Rice Plants and the Possible Role of Aquaporins Plant Cell Physiol., September 1, 2008; 49(9): 1294 - 1305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Azad, M. Katsuhara, Y. Sawa, T. Ishikawa, and H. Shibata Characterization of Four Plasma Membrane Aquaporins in Tulip Petals: A Putative Homolog is Regulated by Phosphorylation Plant Cell Physiol., August 1, 2008; 49(8): 1196 - 1208. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F. Ma, N. Yamaji, N. Mitani, X.-Y. Xu, Y.-H. Su, S. P. McGrath, and F.-J. Zhao Transporters of arsenite in rice and their role in arsenic accumulation in rice grain PNAS, July 22, 2008; 105(29): 9931 - 9935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Norton, D. E. Lou-Hing, A. A. Meharg, and A. H. Price Rice-arsenate interactions in hydroponics: whole genome transcriptional analysis J. Exp. Bot., May 2, 2008; (2008) ern097v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mahdieh, A. Mostajeran, T. Horie, and M. Katsuhara Drought Stress Alters Water Relations and Expression of PIP-Type Aquaporin Genes in Nicotiana tabacum Plants Plant Cell Physiol., May 1, 2008; 49(5): 801 - 813. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Suzuki, K. Nagasuga, and M. Okada The Chilling Injury Induced by High Root Temperature in the Leaves of Rice Seedlings Plant Cell Physiol., March 1, 2008; 49(3): 433 - 442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Sakurai, A. Ahamed, M. Murai, M. Maeshima, and M. Uemura Tissue and Cell-Specific Localization of Rice Aquaporins and Their Water Transport Activities Plant Cell Physiol., January 1, 2008; 49(1): 30 - 39. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Y. Jang, J. Y. Rhee, D. G. Kim, G. C. Chung, J. H. Lee, and H. Kang Ectopic Expression of a Foreign Aquaporin Disrupts the Natural Expression Patterns of Endogenous Aquaporin Genes and Alters Plant Responses to Different Stress Conditions Plant Cell Physiol., September 1, 2007; 48(9): 1331 - 1339. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-G. Choi and D. M. Roberts Arabidopsis NIP2;1, a Major Intrinsic Protein Transporter of Lactic Acid Induced by Anoxic Stress J. Biol. Chem., August 17, 2007; 282(33): 24209 - 24218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Wei, E. Alexandersson, D. Golldack, A. J. Miller, P. O. Kjellbom, and W. Fricke HvPIP1;6, a Barley (Hordeum vulgare L.) Plasma Membrane Water Channel Particularly Expressed in Growing Compared with Non-Growing Leaf Tissues Plant Cell Physiol., August 1, 2007; 48(8): 1132 - 1147. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Koizumi, T. Ookawa, H. Satoh, and T. Hirasawa A Wilty Mutant of Rice has Impaired Hydraulic Conductance Plant Cell Physiol., August 1, 2007; 48(8): 1219 - 1228. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Li, W. Xu, W. Yang, Z. Kong, and Y. Xue Genome-Wide Gene Expression Profiling Reveals Conserved and Novel Molecular Functions of the Stigma in Rice Plant Physiology, August 1, 2007; 144(4): 1797 - 1812. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Yamaji and J. F. Ma Spatial Distribution and Temporal Variation of the Rice Silicon Transporter Lsi1 Plant Physiology, March 1, 2007; 143(3): 1306 - 1313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Martinoia, M. Maeshima, and H. E. Neuhaus Vacuolar transporters and their essential role in plant metabolism J. Exp. Bot., January 1, 2007; 58(1): 83 - 102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. AROCA, A. FERRANTE, P. VERNIERI, and M. J. CHRISPEELS Drought, Abscisic Acid and Transpiration Rate Effects on the Regulation of PIP Aquaporin Gene Expression and Abundance in Phaseolus vulgaris Plants Ann. Bot., December 1, 2006; 98(6): 1301 - 1310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mizutani, S. Watanabe, T. Nakagawa, and M. Maeshima Aquaporin NIP2;1 is Mainly Localized to the ER Membrane and Shows Root-Specific Accumulation in Arabidopsis thaliana Plant Cell Physiol., October 1, 2006; 47(10): 1420 - 1426. [Abstract] [Full Text] [PDF]
|
|