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Plant and Cell Physiology Advance Access originally published online on July 14, 2005
Plant and Cell Physiology 2005 46(9):1494-1504; doi:10.1093/pcp/pci162
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Brassinosteroids Regulate Plasma Membrane Anion Channels in Addition to Proton Pumps During Expansion of Arabidopsis thaliana Cells

Zongshen Zhang1, Javier Ramirez2, David Reboutier1, Mathias Brault1, Jacques Trouverie1, Anne-Marie Pennarun1, Zahia Amiar1, Bernadette Biligui1, Lydia Galagovsky2 and Jean-Pierre Rona1,*

1 Laboratoire d’Electrophysiologie des Membranes, EA 3514, Université Paris 7, 2 Place Jussieu, 75251 Paris Cedex 05, France
2 Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina

* Corresponding author: E-mail, jprona{at}ccr.jussieu.fr; Fax, +33-1-44-27–78–13.

Brassinosteroids (BRs) are involved in numerous physiological processes associated with plant development and especially with cell expansion. Here we report that two BRs, 28-homobrassinolide (HBL) and its direct precursor 28-homocastasterone (HCS), promote cell expansion of Arabidopsis thaliana suspension cells. We also show that cell expansions induced by HBL and HCS are correlated with the amplitude of the plasma membrane hyperpolarization they elicited. HBL, which promoted the larger cell expansion, also provoked the larger hyperpolarization. We observed that membrane hyperpolarization and cell expansion were partially inhibited by the proton pump inhibitor erythrosin B, suggesting that proton pumps were not the only ion transport system modulated by the two BRs. We used a voltage clamp approach in order to find the other ion transport systems involved in the PM hyperpolarization elicited by HBL and HCS. Interestingly, while anion currents were inhibited by both HBL and HCS, outward rectifying K+ currents were increased by HBL but inhibited by HCS. The different electrophysiological behavior shown by HBL and HCS indicates that small changes in the BR skeleton might be responsible for changes in bioactivity.

(Received May 13, 2005; Accepted July 5, 2005)
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