Plant and Cell Physiology Advance Access originally published online on June 17, 2005
Plant and Cell Physiology 2005 46(9):1452-1461; doi:10.1093/pcp/pci155
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
An Arabidopsis Protein with a Novel Calcium-binding Repeat Sequence Interacts with TONSOKU/MGOUN3/BRUSHY1 Involved in Meristem Maintenance
1 Laboratory of Biochemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601 Japan
2 Faculty of Agriculture, Meijo University, Tenpaku-ku, Nagoya, 468-8502 Japan
* Corresponding author: E-mail, takamasa{at}thaliana.myhome.cx; Fax, +81-52-789-4094.
TONSOKU(TSK)/MGOUN3/BRUSHY1 from Arabidopsis thaliana, which plays an important role in the maintenance of meristem organization, contains an LGN repeat motif similar to that found in animal proteins involved in asymmetric cell division. One protein that interacts with the LGN motif of TSK in a yeast two-hybrid screen, TSK-associating protein 1 (TSA1), contains a 10-fold repeat of a unique 41 amino acid sequence. The repeat sequence, with a glutamic acid–phenylalanine–glutamic acid (EFE) conserved core sequence, is enriched with acidic amino acids. TSA1 also contains an N-terminal putative signal peptide and it interacts with the LGN motif of TSK through a C-terminal region separated from the EFE repeats by a putative membrane-spanning region. The recombinant protein consisting of EFE repeats was rich in 
-helical structure and possessed Ca2+-binding activity. Unlike nuclear localization of TSK, the TSA1 fused with green fluorescent protein (GFP) expressed in tobacco BY-2 cells was localized in small cytoplasmic vesicles during interphase. However, cellular localization of both TSA1–GFP and GFP–TSK changed dynamically during mitosis. In particular, both GFP–TSK and TSA1–GFP were concentrated in limited areas that are close to the ends of spindle microtubules ahead of separating chromatids. These results are discussed in terms of the possible involvement of TSK and TSA1 in mitosis.
3 Present address: Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, 464-8602 Japan.
(Received December 22, 2004; Accepted June 13, 2005)
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Yamada, A. J. Nagano, M. Nishina, I. Hara-Nishimura, and M. Nishimura NAI2 Is an Endoplasmic Reticulum Body Component That Enables ER Body Formation in Arabidopsis thaliana PLANT CELL, September 1, 2008; 20(9): 2529 - 2540. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Nagano, Y. Fukao, M. Fujiwara, M. Nishimura, and I. Hara-Nishimura Antagonistic Jacalin-Related Lectins Regulate the Size of ER Body-Type {beta}-Glucosidase Complexes in Arabidopsis thaliana Plant Cell Physiol., June 1, 2008; 49(6): 969 - 980. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Inagaki, T. Suzuki, M.-a. Ohto, H. Urawa, T. Horiuchi, K. Nakamura, and A. Morikami Arabidopsis TEBICHI, with Helicase and DNA Polymerase Domains, Is Required for Regulated Cell Division and Differentiation in Meristems PLANT CELL, April 1, 2006; 18(4): 879 - 892. [Abstract] [Full Text] [PDF]
|
|