Skip Navigation


Plant and Cell Physiology Advance Access originally published online on May 26, 2005
Plant and Cell Physiology 2005 46(8):1255-1263; doi:10.1093/pcp/pci134
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
46/8/1255    most recent
pci134v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (2)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Tang, W.
Right arrow Articles by Newton, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tang, W.
Right arrow Articles by Newton, R. J.
Agricola
Right arrow Articles by Tang, W.
Right arrow Articles by Newton, R. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

JSPP © 2005

Inducible Antisense-mediated Post-transcriptional Gene Silencing in Transgenic Pine Cells Using Green Fluorescent Protein as a Visual Marker

Wei Tang*, Katherine Kinken and Ronald J. Newton

Department of Biology, Howell Science Complex, East Carolina University, Greenville, NC 27858-4353, USA

* Corresponding author: E-mail, tangw{at}mail.ecu.edu; Fax, +1-252-328-4178.

An inducible post-transcriptional gene silencing (PTGS) system was established in Virginia pine (Pinus virginiana Mill.) cells. This system is based on the activation of an antisense gfp gene construct by a chimeric transcriptional activator GVG (Gal4-binding domain–VP16 activation domain–glucocorticoid receptor fusion) upon application of the inducer to gfp transgenic cell lines. A detailed characterization of the inducible PTGS system in transgenic cell lines demonstrated that this system is stringently controlled. The degree of silencing with this construct could be regulated by the concentration of inducer and the time of treatment. Such transgenic cell lines may provide a useful system to study signaling mechanisms of gene silencing in transgenic pine cells. The inducible system could be a useful tool for functional discovery of novel plant genes.

(Received June 7, 2004; Accepted May 19, 2005)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.