Plant and Cell Physiology Advance Access originally published online on May 20, 2005
Plant and Cell Physiology 2005 46(8):1213-1225; doi:10.1093/pcp/pci130
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Transcriptome Profiling of Vertical Stem Segments Provides Insights into the Genetic Regulation of Secondary Growth in Hybrid Aspen Trees
Department of Forestry, Michigan State University, 126 Natural Resources, East Lansing, MI 48824-1222, USA
* Corresponding author: E-mail, hanky{at}msu.edu; Fax, +1-517-432-1143.
In order to better understand the genetic regulation of secondary growth in hybrid aspen (Populus tremula L.xP. alba L.), we carried out a series of cDNA-amplified fragment length polymorphism (AFLP)-based transcriptome analyses in vertical stem segments that represent a gradient of developmental stages with regard to secondary growth. This approach allowed us to screen >80% of the transcriptome expressed in six samples and identify genes differentially expressed with the progress of secondary growth, in a tissue-specific manner. Of the 76,800 transcript-derived fragments (TDFs) analyzed, 271 TDFs were selected and sequenced based on their differential expression patterns. Many of the xylem-up-regulated genes were involved in cell wall and lignin biosynthesis, while the bark-up-regulated genes had diverse functional roles. About 25% of the xylem-up-regulated TDFs analyzed were involved in the phenylpropanoid biosynthesis pathway, which produces the cell wall polymer lignin and various wood extractives. In addition, many of the TDFs showing secondary xylem-specific expression were annotated as genes not previously reported in Populus, including novel cell death proteins, cytoskeleton-interacting proteins, transporters and putative transcription factors.
1 These authors contributed equally to this work.
2 Present address: Danforth Plant Science Research Center, 975 N. Warson Rd., St. Louis, MO 63132, USA.
The nucleotide sequences reported in this paper have been submitted to GenBank under accession numbers CF216193–CF216471.
(Received September 22, 2004; Accepted May 16, 2005)
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