Skip Navigation


Plant and Cell Physiology Advance Access originally published online on May 16, 2005
Plant and Cell Physiology 2005 46(8):1202-1212; doi:10.1093/pcp/pci129
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
46/8/1202    most recent
pci129v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (7)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Buzas, D. M.
Right arrow Articles by Gresshoff, P. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Buzas, D. M.
Right arrow Articles by Gresshoff, P. M.
Agricola
Right arrow Articles by Buzas, D. M.
Right arrow Articles by Gresshoff, P. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

JSPP © 2005

Promoter trapping in Lotus japonicus reveals novel root and nodule GUS expression domains

Diana Mihaela Buzas1,*, Dasharath Lohar2, Shusei Sato3, Yasukazu Nakamura3, Satoshi Tabata3, Claudia Estelle Vickers1,4, Jiri Stiller1 and Peter Michael Gresshoff1

1 ARC Centre of Excellence for Integrative Legume Research, The University of Queensland, 4072 Australia
2 Department of Plant Biology, 250 Bio Sciences Center, 1445 Gortner Avenue, Saint Paul, MN 55108-1095, USA
3 Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba, 292-0812, Japan
4 Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, UK

* Corresponding author: E-mail, d.buzas{at}uq.edu.au; Fax, +61-3365-3556.

Agrobacterium-based transformation was used to introduce a promoter-less glucuronidase uidA gene (ß-glucuronidase; GUS) into Lotus japonicus. Transgenic plants were screened for GUS activation at different stages after inoculation with its symbiont, Mesorhizobium loti. Functional GUS fusion frequencies ranged from about 2 to 5% of the total number of transgenic lines. These lines provide excellent histological markers for tissue ontogeny analysis. Some of the activations generated GUS expression patterns that correspond to well-known tissue types, such as lateral root and nodule primordia, root tips and developing nodules (line CHEETAH). Others generated GUS activation associated with predictable but previously unknown (i) tissue types, such as the vascular bundle of the nodule (line VASCO); or (ii) expression domains, such as pericycle, nodule primordia, nodule and flower connective/vascular tissue (line FATA MORGANA) or inner root cortex cells in the vicinity of a curled root hair, nodule primordia and nodule cortex (line TIMPA). Putative members of two gene superfamilies, EH (Esp homolog) and AAA ATPase (ATPase associated with various cellular activities), were located next to the CHEETAH and VASCO insertions, respectively, and a nodulin gene, LjENOD40–2, was located next to the FATA MORGANA insertion. We utilized promoter GUS fusions to investigate the genetic regulation of LjENOD40–2 and FATA MORGANA GUS. The LjENOD40–2 promoter defined a novel expression domain and the FATA MORGANA nodule expression was reiterated by the 2 kb sequence upstream of the T-DNA insertion.

The nucleotide sequences reported in this paper have been submitted to GenBank under the following accession numbers: LjT22D23-AP008242 (FATA MORGANA), LjT39G20 a and b-AP008240 and AP008241 (CHEETAH), LjT07P16-AP008243 (VASCO).

(Received February 23, 2005; Accepted May 12, 2005)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.