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Plant and Cell Physiology Advance Access originally published online on April 19, 2005
Plant and Cell Physiology 2005 46(7):1149-1153; doi:10.1093/pcp/pci112
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JSPP © 2005

Short Communication

A Resource of 5,814 Dissociation Transposon-tagged and Sequence-indexed Lines of Arabidopsis Transposed from Start Loci on Chromosome 5

Takuya Ito1, Reiko Motohashi2, Takashi Kuromori2, Yoshiteru Noutoshi1, Motoaki Seki1,2, Asako Kamiya2, Saho Mizukado1, Tetsuya Sakurai2,3 and Kazuo Shinozaki1,2,*

1 Plant Molecular Biology Laboratory, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba, 305-0074 Japan
2 Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045 Japan
3 Genomic Knowledge Base Research Team, Bioinfomatics Group, RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045 Japan

* Corresponding author: E-mail, sinozaki{at}rtc.riken.jp; Fax, +81-298-36-9060.

We report here the generation of an additional collection of Dissociation (Ds) transposon-tagged, sequence-indexed lines of Arabidopsis thaliana. Our RIKEN Ds insertion collection now totals 17,668 lines. Our collection has preferential insertions in chromosomes 1 and 5, because Ds was transposed from start loci on those chromosomes (11,854 and 5,814 lines, respectively). We describe here features of the latter 5,814 lines. The former 11,854 lines have been described previously. We have created a searchable database of the insertion sites and mutated genes (http://rarge.gsc.riken.jp/), and are depositing these lines in the RIKEN BioResource Center (http://www.brc.riken.go.jp/lab/epd/Eng/). Our collection of these mutants will contribute to progress in functional genomics of plants.

(Received January 13, 2005; Accepted April 15, 2005)
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