Skip Navigation


Plant and Cell Physiology Advance Access originally published online on April 13, 2005
Plant and Cell Physiology 2005 46(6):923-930; doi:10.1093/pcp/pci098
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
46/6/923    most recent
pci098v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (16)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Wang, J. W.
Right arrow Articles by Wu, J. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, J. W.
Right arrow Articles by Wu, J. Y.
Agricola
Right arrow Articles by Wang, J. W.
Right arrow Articles by Wu, J. Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

JSPP © 2005

Nitric Oxide is Involved in Methyl Jasmonate-induced Defense Responses and Secondary Metabolism Activities of Taxus Cells

Jian Wen Wang1,2 and Jian Yong Wu1,3

1 Department of Applied Biology & Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong
2 Department of Pharmacy, Soochow University, Suzhou, China

3 Corresponding author: E-mail, bcjywu{at}polyu.edu.hk; Fax, +852-23649932.

Methyl jasmonate (MeJA), a methyl ester of jasmonic acid (JA), is a well-established signal molecule in plant defense responses and an effective inducer of secondary metabolite accumulation in plant cell cultures such as the valuable anticancer diterpenoid taxol (paclitaxel) in Taxus spp. This work examines the involvement of nitric oxide (NO) in MeJA-induced plant defense responses and secondary metabolism in Taxus chinensis cell cultures. Exogenously supplied MeJA at 100 µM induced rapid production of NO in the Taxus cell cultures, reaching a maximum within 6 h of MeJA supply. Several other responses occurred concomitantly, including the production of hydrogen peroxide (H2O2), and the increases in intracellular malondialdehyde (MDA) content, lipoxygenase (LOX) and phenylalanine ammonium-lyase (PAL) activities. The MeJA-induced H2O2 production was suppressed by an NO donor, sodium nitroprusside (SNP), but enhanced by NO inhibitors, N {omega}-nitro-l-arginine (l-NNA) and 2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide (PTIO). In contrast, the MeJA-induced MDA, LOX and PAL were all enhanced by the NO donor but suppressed by the NO inhibitors. The NO inhibitors also suppressed MeJA-induced taxol accumulation. These results are suggestive of a role for NO as a signal element for activating the MeJA-induced defense responses and secondary metabolism activities of plant cells.

(Received November 27, 2004; Accepted March 25, 2005)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
J. M. Barrero, M. J. Talbot, R. G. White, J. V. Jacobsen, and F. Gubler
Anatomical and Transcriptomic Studies of the Coleorhiza Reveal the Importance of This Tissue in Regulating Dormancy in Barley
Plant Physiology, June 1, 2009; 150(2): 1006 - 1021.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Rodriguez-Serrano, M. C. Romero-Puertas, D. M. Pazmino, P. S. Testillano, M. C. Risueno, L. A. del Rio, and L. M. Sandalio
Cellular Response of Pea Plants to Cadmium Toxicity: Cross Talk between Reactive Oxygen Species, Nitric Oxide, and Calcium
Plant Physiology, May 1, 2009; 150(1): 229 - 243.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S.-J. Wu and J.-Y. Wu
Extracellular ATP-induced NO production and its dependence on membrane Ca2+ flux in Salvia miltiorrhiza hairy roots
J. Exp. Bot., October 1, 2008; 59(14): 4007 - 4016.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
L. Zhang and D. Xing
Methyl Jasmonate Induces Production of Reactive Oxygen Species and Alterations in Mitochondrial Dynamics that Precede Photosynthetic Dysfunction and Subsequent Cell Death
Plant Cell Physiol., July 1, 2008; 49(7): 1092 - 1111.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
S.-J. Wu, Y.-S. Liu, and J.-Y. Wu
The Signaling Role of Extracellular ATP and its Dependence on Ca2+ Flux in Elicitation of Salvia miltiorrhiza Hairy Root Cultures
Plant Cell Physiol., April 1, 2008; 49(4): 617 - 624.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. C. Palmieri, S. Sell, X. Huang, M. Scherf, T. Werner, J. Durner, and C. Lindermayr
Nitric oxide-responsive genes and promoters in Arabidopsis thaliana: a bioinformatics approach
J. Exp. Bot., February 13, 2008; (2008) erm345v1.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
M. M. Izaguirre, C. A. Mazza, A. SvatoS, I. T. Baldwin, and C. L. BallarE
Solar Ultraviolet-B Radiation and Insect Herbivory Trigger Partially Overlapping Phenolic Responses in Nicotiana attenuata and Nicotiana longiflora
Ann. Bot., January 1, 2007; 99(1): 103 - 109.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
S. Saito, A. Yamamoto-Katou, H. Yoshioka, N. Doke, and K. Kawakita
Peroxynitrite Generation and Tyrosine Nitration in Defense Responses in Tobacco BY-2 Cells
Plant Cell Physiol., June 1, 2006; 47(6): 689 - 697.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.