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Plant and Cell Physiology Advance Access originally published online on March 28, 2005
Plant and Cell Physiology 2005 46(6):817-826; doi:10.1093/pcp/pci096
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JSPP © 2005

Rapid Paper

Regulation of CAPRICE Transcription by MYB Proteins for Root Epidermis Differentiation in Arabidopsis

Yoshihiro Koshino-Kimura1,2, Takuji Wada2, Tatsuhiko Tachibana1,4, Ryuji Tsugeki1, Sumie Ishiguro1,5 and Kiyotaka Okada1,2,3,6

1 Department of Botany, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto, 606-8502 Japan
2 Plant Science Center, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045 Japan
3 Core Research for Evolutional Science and Technology (CREST) Research Project, Japan Science and Technology Agency, Kawaguchi, Saitama, 322-0012, Japan

6 Corresponding author: kiyo{at}ok-lab.bot.kyoto-u.ac.jp; Fax, +81-75-753-4257.

Epidermal cell differentiation in Arabidopsis root is studied as a model system for understanding cell fate specification. Two types of MYB-related transcription factors are involved in this cell differentiation. One of these, CAPRICE (CPC), encoding an R3-type MYB protein, is a positive regulator of hair cell differentiation and is preferentially transcribed in hairless cells. We analyzed the regulatory mechanism of CPC transcription. Deletion analyses of the CPC promoter revealed that hairless cell-specific transcription of the CPC gene required a 69 bp sequence, and a tandem repeat of this region was sufficient for its expression in epidermis. This region includes two MYB-binding sites, and the epidermis-specific transcription of CPC was abolished when base substitutions were introduced in these sites. We showed by gel mobility shift experiments and by yeast one-hybrid assay that WEREWOLF (WER), which is an R2R3-type MYB protein, directly binds to this region. We showed that WER also binds to the GL2 promoter region, indicating that WER directly regulates CPC and GL2 transcription by binding to their promoter regions.

4 Present address: Chugai Pharmaceutical Co., Ltd., 1-135 Komakado, Gotemba, Shizuoka, 412-8513 Japan.

5 Present address: Department of Cellular Mechanisms and Functions, Graduate School of Bio-Agricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan.

(Received February 21, 2005; Accepted March 23, 2005)
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