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Plant and Cell Physiology Advance Access originally published online on March 7, 2005
Plant and Cell Physiology 2005 46(5):790-796; doi:10.1093/pcp/pci073
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JSPP © 2005

Short Communication

Analysis of the Function of the Photoreceptors Phytochrome B and Phytochrome D in Nicotiana plumbaginifolia and Arabidopsis thaliana

Aurora Piñas Fernández1,3, Patricia Gil1,3, Ildiko Valkai2, Ferenc Nagy2 and Eberhard Schäfer1,4

1 Albert-Ludwigs-Universität Freiburg, Institut für Biologie II/ Botanik, Schänzlestraße 1, 79104 Freiburg, Germany
2 Institute of Plant Biology, Biological Research Centre, Temesvári krt 62, H-6726 Szeged, Hungary

4 Corresponding author: E-mail, eberhard.schaefer{at}biologie.uni-freiburg.de; Fax, +49-761-2032629.

To investigate the mechanism of phytochrome action in vivo, NtPHYB, AtPHYB and phyD:green fluorescent protein (GFP) were overexpressed in Nicotiana plumbaginifolia and Arabidopsis thaliana. The expression of 35S:NtPHYB:GFP and 35S:AtPHYB:GFP complemented the tobacco hgl2 and Arabidopsis phyB-9 mutations, whereas the 35S:AtPHYD:GFP only rescued the hgl2 mutant. All three fusion proteins are transported into the nucleus in all genetic backgrounds. These data indicate that AtPHYD:GFP is biologically active and functions as the main red light receptor in transgenic tobacco, and establish an experimental system for the functional analysis of this elusive photoreceptor in vivo.

3 These authors contributed equally to this work.

(Received September 14, 2004; Accepted February 11, 2005 )
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