Plant and Cell Physiology Advance Access originally published online on March 7, 2005
Plant and Cell Physiology 2005 46(5):754-761; doi:10.1093/pcp/pci078
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Differential Expression Pattern of C4 Bundle Sheath Expression Genes in Rice, a C3 Plant
1 Faculty of Agriculture, Kagawa University, Miki, Kita, Kagawa, 761-0795 Japan
2 Japan Tobacco Inc., 700 Higashibara, Toyoda, Iwata, Shizuoka, 438 Japan
3 Japan Turfgrass Inc., 1-1-20, Higashi-nihonbashi, Chuo, Tokyo, 103-0004 Japan
4 National Institute of Agrobiological Resources, Tsukuba, 305-8602 Japan
5 BioScience Center, Nagoya University, Chikusa, Nagoya, 464-8601 Japan
6 Corresponding author: E-mail, nomura{at}ag.kagawa-u.ac.jp; Fax, +81-87-891-3021.
NADP-malic enzyme (NADP-ME) and phosphoenolpyruvate carboxykinase (PCK) are specifically expressed in bundle sheath cells (BSCs) in NADP-ME-type and PCK-type C4 plants, respectively. Unlike the high activities of these enzymes in the green leaves of C4 plants, their low activities have been detected in the leaves of C3 plants. In order to elucidate the differences in the gene expression system between C3 and C4 plants, we have produced chimeric constructs with the ß-glucuronidase (GUS) reporter gene under the control of the maize NADP-Me (ZmMe) or Zoysia japonica Pck (ZjPck) promoter and introduced these constructs into rice. In leaves of transgenic rice, the ZmMe promoter directed GUS expression not only in mesophyll cells (MCs) but also in BSCs and vascular cells, whereas the ZjPck promoter directed GUS expression only in BSCs and vascular cells. Neither the ZjPck nor ZmMe promoters induced GUS expression due to light. In rice leaves, the endogenous NADP-Me (OsMe1) was expressed in MCs, BSCs and vascular cells, whereas the rice Pck (OsPck1) was expressed only in BSCs and vascular cells. Taken together, the results obtained from transgenic rice demonstrate that the expression pattern of ZmMe or ZjPck in transgenic rice was reflected by that of its counterpart gene in rice.
(Received August 8, 2004; Accepted February 20, 2005
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