Skip Navigation


Plant and Cell Physiology Advance Access originally published online on February 2, 2005
Plant and Cell Physiology 2005 46(4):666-670; doi:10.1093/pcp/pci064
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
46/4/666    most recent
pci064v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (4)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Sasaki, N.
Right arrow Articles by Ozeki, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sasaki, N.
Right arrow Articles by Ozeki, Y.
Agricola
Right arrow Articles by Sasaki, N.
Right arrow Articles by Ozeki, Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

JSPP © 2005

Short Communication

Isolation and Characterization of cDNAs Encoding an Enzyme with Glucosyltransferase Activity for cyclo-DOPA from Four O’clocks and Feather Cockscombs

Nobuhiro Sasaki1, Katsuhiro Wada2, Takatoshi Koda2, Kichiji Kasahara3, Taiji Adachi4 and Yoshihiro Ozeki1,5

1 Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588 Japan
2 San-Ei Gen F.F.I., Inc., 1-1-11 Sanwa-machi, Toyonaka, Osaka, 561-8588 Japan
3 1128 Moro-oka, Kohoku-ku, Yokohama, Kanagawa, 222-0002 Japan
4 Department of Plant Science, College of Agriculture, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka, 599-8531 Japan

5 Corresponding author: E-mail, ozeky{at}cc.tuat.ac.jp; Fax, +81-42-388-7239.

cDNAs encoding an enzyme with UDP-glucose:cyclo-DOPA 5-O-glucosyltransferase activity were isolated from four o’clocks and feather cockscombs. Phylogenetic analysis of the amino acid sequences deduced from the cDNAs show that they represent a single subclade distinct from those of other phenylpropanoid and flavonoid glucosyltransferases. Changes in the amount of transcripts of the cDNA in four o’clocks correlated with the accumulation of betanin during flower development. The cDNAs isolated here were candidates for the gene of the enzyme involved in another pathway of betacyanin biosynthesis via glucosylation at the cyclo-DOPA step rather than at the betanidin step.

The nucleotide sequences reported in this paper have been submitted to the DDBJ under accession numbers MjcDOPA5GT cDNA, AB182643; CccDOPA5GT cDNA, AB182644.

Received August 19, 2004; Accepted January 23, 2005
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.