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Plant and Cell Physiology Advance Access originally published online on February 2, 2005
Plant and Cell Physiology 2005 46(3):489-496; doi:10.1093/pcp/pci047
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JSPP © 2005

High Intracellular Phosphorus Contents Exhibit a Correlation with Arsenate Resistance in Chlamydomonas Mutants

Isao Kobayashi1,4, Shoko Fujiwara1,2, Kosuke Shimogawara3, Chiseko Sakuma1, Yasuo Shida1, Toshikazu Kaise1, Hideaki Usuda3 and Mikio Tsuzuki1,2,5

1 School of Life Science, Tokyo University of Pharmacy and Life Science, Horinouchi, Hachioji, Tokyo, 192-0392 Japan
2 CREST, Japan Science and Technology Agency, Kawaguchi, Saitama, 332-0392 Japan
3 Laboratory of Chemistry, Teikyo University School of Medicine, Ohtsuka, Hachioji, Tokyo, 192-0395 Japan

5 Corresponding author: E-mail, mtsu{at}ls.toyaku.ac.jp; Fax, +81-426-76-6721.

Pi in the medium relieved the toxicity of arsenate against cellular growth of Chlamydomonas reinhardtii. To investigate the relationship between intracellular P contents and arsenate resistance, we determined the intracellular P contents of arsenate-sensitive and arsenate-resistant mutants, which had been generated by random insertional mutagenesis. All 13 arsenate-resistant mutants showed higher P contents than the parent strain, while arsenate-sensitive mutants with high P contents were not found. In one of the arsenate-resistant mutants, AR3, the intracellular P content was about twice that in the wild type during growth in the absence of arsenate. Arsenate incorporation in AR3 was suppressed within 10 min after the addition of 1 mM arsenate, while Pi incorporation continued even after arsenate uptake ceased. Whereas the P content of the wild type decreased to half in the presence of 0.5 mM arsenate, almost the same degree (about 50%) of decrease was observed in AR3 cells grown in the presence of as much as 3 mM arsenate. AR3, in which PTB1, a homolog of a Pi transporter gene, had been disrupted, exhibited a higher activity of a high-affinity Pi transporter, suggesting that it may be due to a compensatory transport activity. These data suggest that the intracellular level of P is one of the important factors of arsenate resistance.

4 Present address: Insect Biology and Sericology Department, National Institute of Agrobiological Sciences, Owashi, Tsukuba, Ibaraki, 305–8634 Japan.

Received August 19, 2004; Accepted December 27, 2004
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