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Plant and Cell Physiology Advance Access originally published online on February 2, 2005
Plant and Cell Physiology 2005 46(3):454-466; doi:10.1093/pcp/pci042
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JSPP © 2005

Metabolic Flux Analysis of the Phenylpropanoid Pathway in Elicitor-treated Potato Tuber Tissue

Fumio Matsuda1,3, Keiko Morino1,3, Rieko Ano1, Masaki Kuzawa1, Kyo Wakasa2,3 and Hisashi Miyagawa1,3,4

1 Division of Applied Life Sciences, Department of Agriculture, Kyoto University, Kyoto, 606-8502 Japan
2 Biotechnology Laboratory, National Institute of Crop Science, Tsukuba, 305-8515 Japan
3 Plant Functions and their Control, CREST, Japan Science and Technology Agency, Tokyo, 103-0027 Japan

4 Corresponding author: E-mail, miyagawa{at}kais.kyoto-u.ac.jp; Fax, +81-75-753-6123.

The effects of ß-1,3-oligosaccharide elicitor on the metabolism of phenylpropanoids in potato tuber were analyzed quantitatively, by monitoring the time-dependent changes in the levels of seven compounds. The elicitor treatment caused an increase in the pool size of octopamine and tyramine amides (N-p-coumaroyloctopamine, N-feruloyloctopamine, N-p-coumaroyltyramine and N-feruloyltyramine), as well as a decrease in that of chlorogenic acid and putrescine amides (caffeoylputrescine and feruloylputrescine). An analysis of metabolic flux using stable isotope labeling and liquid chromatography–spectrometry (LC–MS) detection clearly demonstrated that the changes in the pool size of these compounds were correlated with the changes in their flux for biosynthesis (J in) upon elicitor treatment. The increase in J in in the cases of octopamine and tyramine amides was accompanied by an increase in flux for the transformation (J out), indicating a rapid turnover of these compounds in the elicitor-treated tuber tissue. The result of the flux analysis indicated that the actual activation of the biosynthesis of octopamine and tyramine amides after the elicitor treatment was greater than that estimated from the changes in their levels in the potato tissue. These findings suggest that these amide compounds and their metabolic derivatives play an important role in the defense-related metabolism of phenylpropanoids in potato.

Received August 22, 2004; Accepted December 24, 2004
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