Skip Navigation


Plant and Cell Physiology Advance Access originally published online on February 2, 2005
Plant and Cell Physiology 2005 46(2):381-386; doi:10.1093/pcp/pci036
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
46/2/381    most recent
pci036v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (3)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Oki, K.
Right arrow Articles by Iwasaki, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oki, K.
Right arrow Articles by Iwasaki, Y.
Agricola
Right arrow Articles by Oki, K.
Right arrow Articles by Iwasaki, Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

JSPP © 2005

Short Communication

Study of the Constitutively Active Form of the {alpha} Subunit of Rice Heterotrimeric G Proteins

Katsuyuki Oki, Yukiko Fujisawa, Hisaharu Kato and Yukimoto Iwasaki1

Fukui Prefectural University, Faculty of Bioscience, Kenjyojima, Matsuoka-cho, Yoshida-gun, Fukui, 910-1195 Japan

1 Corresponding author: E-mail, iwasaki{at}fpu.ac.jp; Fax, +81-776-61-6015.

We used site-directed mutagenesis to engineer two constitutively active forms of the {alpha} subunit of a rice heterotrimeric G protein. The recombinant proteins produced from these novel cDNAs had GTP-binding activity but no GTPase activity. A chimeric gene for a constitutively active form of the {alpha} subunit was introduced into the rice mutant d1, which is defective for the {alpha}-subunit gene. All the transformants essentially showed a wild-type phenotype compared with normal cultivars, although seed sizes were substantially increased and internode lengths also showed some increase.

Received August 25, 2004; Accepted December 8, 2004
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
Y. Trusov, W. Zhang, S. M. Assmann, and J. R. Botella
G{gamma}1 + G{gamma}2 != G{beta}: Heterotrimeric G Protein G{gamma}-Deficient Mutants Do Not Recapitulate All Phenotypes of G{beta}-Deficient Mutants
Plant Physiology, June 1, 2008; 147(2): 636 - 649.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.