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Plant and Cell Physiology Advance Access originally published online on February 2, 2005
Plant and Cell Physiology 2005 46(2):375-380; doi:10.1093/pcp/pci023
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JSPP © 2005

Short Communication

Phosphorylation of Sphingoid Long-chain Bases in Arabidopsis: Functional Characterization and Expression of the First Sphingoid Long-chain Base Kinase Gene in Plants

Hiroyuki Imai1 and Hideki Nishiura

Department of Biology, Graduate School of Natural Science, Konan University, Kobe, 658-8501 Japan

1 Corresponding author: E-mail, imai{at}konan-u.ac.jp; Fax, +81-78-4352539.

Sphingoid long-chain base (LCB) kinase catalyzes the phosphorylation of LCBs to form LCB 1-phosphates. Based on sequence identity to murine sphingosine kinase (mSPHK1), we cloned and characterized the first plant LCB kinase gene from Arabidopsis (AtLCBK1). Using recombinant AtLCBK1 protein from Escherichia coli cells, we confirmed that the enzyme specifically phosphorylated d-erythro-dihydrosphingosine (DHS), but not N-acetyl-DHS or d-threo-DHS. AtLCBK1 also phosphorylated d-erythro-sphingosine, trans-4, trans-8-sphingadienine and phytosphingosine. We found that AtLCBK1 mRNA is highly expressed in flowers. AtLCBK1 transcripts were slightly increased by low humidity or abscisic acid treatments, suggesting that AtLCBK1 is constitutively expressed under these treatments.

The nucleotide sequence reported in this paper has been submitted to the DDBJ, EMBL and GenBank databases under the accession number AB046717.

Received August 30, 2004; Accepted November 18, 2004
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