Plant and Cell Physiology Advance Access originally published online on February 2, 2005
Plant and Cell Physiology 2005 46(2):367-374; doi:10.1093/pcp/pci037
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Phototropin from Chlamydomonas reinhardtii is Functional in Arabidopsis thaliana
1 Department of Botany, Graduate School of Science, Kyoto University, Kitashirakawa-Oiwake-Cho, Sakyo-Ku, Kyoto, 606-8502 Japan
2 Research and Development Center for Higher Education, Faculty of Science, Kyushu University, Ropponmatsu, Fukuoka, 810-8560 Japan
3 Department of Biology, Faculty of Science, Kyushu University, Ropponmatsu, Fukuoka, 810-8560 Japan
4 Department of Plant Biology, Carnegie Institution of Washington, Stanford, CA 94305, U.S.A.
6 Corresponding author: E-mail, nagatani{at}physiol.bot.kyoto-u.ac.jp; Fax, +81-75753-4126.
Phototropin, a plant blue light photoreceptor, mediates important blue light responses such as phototropism, chloroplast positioning and stomatal opening in higher plants. In Arabidopsis thaliana, two phototoropins, phototropin 1 and 2, are known. Recently, in the unicellular green alga, Chlamydomonas reinhardtii, a phototropin homolog was identified. It exhibits photochemical properties similar to those of higher plant phototropins and is involved in multiple steps of the sexual life cycle of Chlamydomonas. Here, we expressed Chlamydomonas phototropin in Arabidopsis to examine whether it is active in a distantly related plant species. The Arabidopsis mutant deficient in both phototropin 1 and 2 was transformed with a vector containing Chlamydomonas phototropin cDNA fused to a cauliflower mosaic virus 35S promoter. The resulting lines were classified into high, medium and low expressers based on RNA gel blot and immunoblot analyses. Typical phototropin responses were restored in high expression lines. These results demonstrate that Chlamydomonas phototropin is functional in higher plants. Hence, the basic mechanism of phototropin action is highly conserved, even though its apparent physiological functions are quite diverse.
5 Present address: Plant Science Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, University Avenue, Glasgow G12 8QQ, U.K.
Received September 22, 2004; Accepted December 15, 2004
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