Plant and Cell Physiology Advance Access originally published online on February 2, 2005
Plant and Cell Physiology 2005 46(2):300-311; doi:10.1093/pcp/pci031
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Indirect ABA-dependent Regulation of Seed Storage Protein Genes by FUSCA3 Transcription Factor in Arabidopsis
1 Life Science Research Center, Mie University, Tsu, 514-8507 Japan
2 Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, 464-8601 Japan
3 Corresponding author: E-mail, hattori{at}agr.nagoya-u.ac.jp; Fax, +81-52-789-5214.
The key transcription factors that control seed maturation, ABSCISIC ACID INSENSITIVE3 (ABI3) and FUSCA3 (FUS3), share homologous DNA-binding domains. Regulation of seed storage protein genes At2S3 and CRC by ABI3 and FUS3 was investigated using transgenic plants in which ABI3 and FUS3 could be ectopically induced by steroid hormones. Like ABI3, the presence of FUS3 led to expression of At2S3 and CRC in vegetative tissues. FUS3-mediated induction of CRC was completely dependent on exogenous abscisic acid (ABA), while At2S3 was weakly induced without ABA but strongly enhanced with ABA. This ABA dependency of FUS3-induced CRC and At2S3 expression was similar to that observed for ABI3. However, kinetic analysis revealed distinctions between the mechanisms of ABA-dependent CRC regulation by FUS3 or ABI3, and between target genes. While At2S3 activation by FUS3 was rapid, CRC induction by FUS3 in the presence of ABA, and by ABA followed by the presence of FUS3, took a significantly longer time (24–36 h). This suggested the involvement of an indirect mechanism requiring the ABA- and FUS3-dependent synthesis of intermediate regulatory factor(s). A chimeric protein composed of the FUS3 B3 domain, and a heterologous activation domain and nuclear localization signal exhibited a tight coupling with ABA regulation as observed for wild-type FUS3. Simultaneous induction of FUS3 and ABI3 did not result in the synergistic activation of CRC and At2S3. Based on these results, similarities and differences in the mechanisms of seed storage protein gene regulation by FUS3 and ABI3 are discussed.
Received June 10, 2004; Accepted November 24, 2004
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Mu, H. Tan, Q. Zheng, F. Fu, Y. Liang, J. Zhang, X. Yang, T. Wang, K. Chong, X.-J. Wang, et al. LEAFY COTYLEDON1 Is a Key Regulator of Fatty Acid Biosynthesis in Arabidopsis Plant Physiology, October 1, 2008; 148(2): 1042 - 1054. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Verdier and R. D. Thompson Transcriptional Regulation of Storage Protein Synthesis During Dicotyledon Seed Filling Plant Cell Physiol., September 1, 2008; 49(9): 1263 - 1271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Tang, A. Hou, M. Babu, V. Nguyen, L. Hurtado, Q. Lu, J. C. Reyes, A. Wang, W. A. Keller, J. J. Harada, et al. The Arabidopsis BRAHMA Chromatin-Remodeling ATPase Is Involved in Repression of Seed Maturation Genes in Leaves Plant Physiology, July 1, 2008; 147(3): 1143 - 1157. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kotak, E. Vierling, H. Baumlein, and P. v. Koskull-Doring A Novel Transcriptional Cascade Regulating Expression of Heat Stress Proteins during Seed Development of Arabidopsis PLANT CELL, January 1, 2007; 19(1): 182 - 195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Braybrook, S. L. Stone, S. Park, A. Q. Bui, B. H. Le, R. L. Fischer, R. B. Goldberg, and J. J. Harada Genes directly regulated by LEAFY COTYLEDON2 provide insight into the control of embryo maturation and somatic embryogenesis PNAS, February 28, 2006; 103(9): 3468 - 3473. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kagaya, R. Toyoshima, R. Okuda, H. Usui, A. Yamamoto, and T. Hattori LEAFY COTYLEDON1 Controls Seed Storage Protein Genes through Its Regulation of FUSCA3 and ABSCISIC ACID INSENSITIVE3 Plant Cell Physiol., March 1, 2005; 46(3): 399 - 406. [Abstract] [Full Text] [PDF]
|
|