Skip Navigation


Plant and Cell Physiology Advance Access originally published online on August 8, 2005
Plant and Cell Physiology 2005 46(10):1666-1673; doi:10.1093/pcp/pci182
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
46/10/1666    most recent
pci182v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (8)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Sivitz, A. B.
Right arrow Articles by Ward, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sivitz, A. B.
Right arrow Articles by Ward, J. M.
Agricola
Right arrow Articles by Sivitz, A. B.
Right arrow Articles by Ward, J. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


Analysis of the Transport Activity of Barley Sucrose Transporter HvSUT1

Alicia B. Sivitz, Anke Reinders and John M. Ward*

Department of Plant Biology, University of Minnesota Twin Cities, 1445 Gortner Avenue, 250 Biological Sciences Center, St. Paul, MN 55108, USA

* Corresponding author: E-mail, jward{at}tc.umn.edu; Fax, +1-612-625-1738.

Localization studies indicate that barley (Hordeum vulgare) sucrose transporter HvSUT1 functions in sucrose uptake into seeds during grain filling. To further understand the physiological function of HvSUT1, we have expressed the HvSUT1 cDNA in Xenopus laevis oocytes and analyzed the transport activity by two-electrode voltage clamping. Consistent with a H+-coupled transport mechanism, sucrose induced large inward currents in HvSUT1-expressing oocytes with a K 0.5 of 3.8 mM at pH 5.0 and a membrane potential of –157 mV. Of 21 other sugars tested, four glucosides were also transported by HvSUT1. These glucosides were maltose, salicin (2-(hydroxymethyl) phenyl ß-d-glucoside), {alpha}-phenylglucoside and {alpha}-paranitrophenylglucoside. Kinetic analysis of transport of these substrates by HvSUT1 was performed and K 0.5 values were measured. The apparent affinity for all substrates was dependent on membrane potential and pH with lower K 0.5 values at lower external pH and more negative membrane potentials. HvSUT1 was more selective for {alpha}-glucosides over ß-glucosides than the Arabidopsis sucrose transporter AtSUC2. Several substrates transported by AtSUC2 (ß-phenylglucoside, ß-paranitrophenylglucoside, {alpha}-methylglucoside, turanose, and arbutin (hydroquinone ß-d-glucoside)) showed low or undetectable transport by HvSUT1. Of these, ß-paranitrophenylglucoside inhibited sucrose transport by HvSUT1 indicating that it interacts with the transporter while arbutin and {alpha}-methyl glucoside did not inhibit. The results demonstrate significant differences in substrate specificity between HvSUT1 and AtSUC2.

(Received March 3, 2005; Accepted July 26, 2005)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
T. L. Slewinski, R. Meeley, and D. M. Braun
Sucrose transporter1 functions in phloem loading in maize leaves
J. Exp. Bot., March 1, 2009; 60(3): 881 - 892.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. M. Braun and T. L. Slewinski
Genetic Control of Carbon Partitioning in Grasses: Roles of Sucrose Transporters and Tie-dyed Loci in Phloem Loading
Plant Physiology, January 1, 2009; 149(1): 71 - 81.
[Full Text] [PDF]

Home page
 J Exp BotHome page
G. N. Scofield, T. Hirose, N. Aoki, and R. T. Furbank
Involvement of the sucrose transporter, OsSUT1, in the long-distance pathway for assimilate transport in rice
J. Exp. Bot., September 1, 2007; 58(12): 3155 - 3169.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. B. Sivitz, A. Reinders, M. E. Johnson, A. D. Krentz, C. P.L. Grof, J. M. Perroux, and J. M. Ward
Arabidopsis Sucrose Transporter AtSUC9. High-Affinity Transport Activity, Intragenic Control of Expression, and Early Flowering Mutant Phenotype
Plant Physiology, January 1, 2007; 143(1): 188 - 198.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. Aoki, G. N. Scofield, X.-D. Wang, C. E. Offler, J. W. Patrick, and R. T. Furbank
Pathway of Sugar Transport in Germinating Wheat Seeds
Plant Physiology, August 1, 2006; 141(4): 1255 - 1263.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.