© 2004 Oxford University Press
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Mutation of a Nitrate Transporter, AtNRT1:4, Results in a Reduced Petiole Nitrate Content and Altered Leaf Development
1 Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan
2 Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan
3 Department of Horticulture, National Chung Hsing University, Taichung, Taiwan
Unlike nitrate uptake of plant roots, less is known at the molecular level about how nitrate is distributed in various plant tissues. In the present study, characterization of the nitrate transporter, AtNRT1:4, revealed a special role of petiole in nitrate homeostasis. Electrophysiological studies using Xenopus oocytes showed that AtNRT1:4 was a low-affinity nitrate transporter. Whole-mount in situ hybridization and RT–PCR demonstrated that AtNRT1:4 was expressed in the leaf petiole. In the wild type, the leaf petiole had low nitrate reductase activity, but a high nitrate content, indicating that it is the storage site for nitrate, whereas, in the atnrt1:4 mutant, the petiole nitrate content was reduced to 50–64% of the wild-type level. Moreover, atnrt1:4 mutant leaves were wider than wild-type leaves. This study revealed a critical role of AtNRT1:4 in regulating leaf nitrate homeostasis, and the deficiency of AtNRT1:4 can alter leaf development.
4 Present address: Department of Biotechnology, China Institute of Technology, Taipei, Taiwan.
5 Present address: Center for Plant Genomics, Iowa State University, Ames, IA 50011, U.S.A.
6 Present address: Department of Life Science, Fu Jen Catholic University, Taipei, Taiwan.
7 Corresponding author: E-mail, mbyftsay{at}ccvax.sinica.edu.tw; Fax, +886-2-27826085.
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